[The formation of monoclonal antibody to the cell membrane region of the stratum corneum and keratohyaline granules of newborn rat].

We have developed a monoclonal antibody to the cell membrane region of the stratum corneum and keratohyalin granules using rat hematoxylin stainable proteins as an antigen. These hematoxylin stainable proteins were partially purified utilizing preparative isoelectric focusing from 50 mM Tris-HCl buffer soluble fraction of newborn rat epidermis. The developed monoclonal antibody located in both sites of the cell membrane region of the stratum corneum and keratohyalin granules (Ted-R-3). In an immunoblotting study using two-dimensional electrophoresis, a single protein which had a molecular weight of 55 kd reacted. The amino acid analysis of the affinity purified antigen showed that glycine, serine and glutamic acid residues comprised 20.4%, 18% and 12.6%, respectively. However, histidine and cystine residues comprised only 2.8% and 0.12%, respectively.