Takahashi M, Tezuka T
Nihon Hifuka Gakkai Zasshi. 1989 Feb;99(2):111-6.
We have developed a monoclonal antibody to the cell membrane region of the stratum corneum and keratohyalin granules using rat hematoxylin stainable proteins as an antigen. These hematoxylin stainable proteins were partially purified utilizing preparative isoelectric focusing from 50 mM Tris-HCl buffer soluble fraction of newborn rat epidermis. The developed monoclonal antibody located in both sites of the cell membrane region of the stratum corneum and keratohyalin granules (Ted-R-3). In an immunoblotting study using two-dimensional electrophoresis, a single protein which had a molecular weight of 55 kd reacted. The amino acid analysis of the affinity purified antigen showed that glycine, serine and glutamic acid residues comprised 20.4%, 18% and 12.6%, respectively. However, histidine and cystine residues comprised only 2.8% and 0.12%, respectively.
我们使用大鼠苏木精可染色蛋白作为抗原，开发了一种针对角质层和透明角质颗粒细胞膜区域的单克隆抗体。这些苏木精可染色蛋白是利用制备性等电聚焦从新生大鼠表皮的50 mM Tris-HCl缓冲液可溶部分中部分纯化得到的。所开发的单克隆抗体定位于角质层和透明角质颗粒的细胞膜区域（Ted-R-3）。在使用二维电泳的免疫印迹研究中，一种分子量为55 kd的单一蛋白质发生了反应。亲和纯化抗原的氨基酸分析表明，甘氨酸、丝氨酸和谷氨酸残基分别占20.4%、18%和12.6%。然而，组氨酸和胱氨酸残基分别仅占2.8%和0.12%。
