Ataollahi Forough, Pramanik Sumit, Moradi Ali, Dalilottojari Adel, Pingguan-Murphy Belinda, Wan Abas Wan Abu Bakar, Abu Osman Noor Azuan
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia.
J Biomed Mater Res A. 2015 Jul;103(7):2203-13. doi: 10.1002/jbm.a.35186. Epub 2014 Apr 25.
Extracellular environments can regulate cell behavior because cells can actively sense their mechanical environments. This study evaluated the adhesion, proliferation and morphology of endothelial cells on polydimethylsiloxane (PDMS)/alumina (Al2 O3 ) composites and pure PDMS. The substrates were prepared from pure PDMS and its composites with 2.5, 5, 7.5, and 10 wt % Al2 O3 at a curing temperature of 50°C for 4 h. The substrates were then characterized by mechanical, structural, and morphological analyses. The cell adhesion, proliferation, and morphology of cultured bovine aortic endothelial (BAEC) cells on substrate materials were evaluated by using resazurin assay and 1,1'-dioctadecyl-1,3,3,3',3'-tetramethylindocarbocyanine perchlorate-acetylated LDL (Dil-Ac-LDL) cell staining, respectively. The composites (PDMS/2.5, 5, 7.5, and 10 wt % Al2 O3 ) exhibited higher stiffness than the pure PDMS substrate. The results also revealed that stiffer substrates promoted endothelial cell adhesion and proliferation and also induced spread morphology in the endothelial cells compared with lesser stiff substrates. Statistical analysis showed that the effect of time on cell proliferation depended on stiffness. Therefore, this study concludes that the addition of different Al2 O3 percentages to PDMS elevated substrate stiffness which in turn increased endothelial cell adhesion and proliferation significantly and induced spindle shape morphology in endothelial cells.
细胞外环境可以调节细胞行为，因为细胞能够主动感知其力学环境。本研究评估了内皮细胞在聚二甲基硅氧烷（PDMS）/氧化铝（Al2O3）复合材料和纯PDMS上的黏附、增殖及形态。这些基底由纯PDMS及其与2.5%、5%、7.5%和10%重量百分比的Al2O3的复合材料在50°C固化温度下制备4小时而成。然后通过力学、结构和形态分析对这些基底进行表征。分别使用刃天青分析法和1,1'-二油酰基-1,3,3,3',3'-四甲基吲哚羰花青高氯酸盐-乙酰化低密度脂蛋白（Dil-Ac-LDL）细胞染色法评估培养的牛主动脉内皮（BAEC）细胞在基底材料上的细胞黏附、增殖及形态。复合材料（PDMS/2.5%、5%、7.5%和10%重量百分比的Al2O3）比纯PDMS基底表现出更高的硬度。结果还表明，与较软的基底相比，较硬的基底促进了内皮细胞的黏附与增殖，并且还诱导内皮细胞呈现铺展形态。统计分析表明时间对细胞增殖的影响取决于硬度。因此，本研究得出结论，向PDMS中添加不同百分比的Al2O3可提高基底硬度，进而显著增加内皮细胞的黏附与增殖，并诱导内皮细胞呈现纺锤形形态。
