Lin Jia-Wei, Tsai Cheng-Chia, Chen Li-Jen, Niu Ho-Shan, Chang Chen Kuei, Niu Chiang-Shan
Department of Neurosurgery, College of Medicine, Taipei Medical University and Shuang Ho Hospital, Taipei Medical University, Taipei City 10361, Taiwan.
Graduate Institute of Disease Prevention and Control, College of Medicine, Taipei Medical University and Shuang Ho Hospital, Taipei Medical University, Taipei City 10361, Taiwan.
Biomed Res Int. 2014;2014:354348. doi: 10.1155/2014/354348. Epub 2014 Mar 9.
Musclin is a novel skeletal muscle-derived factor found in the signal sequence trap of mouse skeletal muscle cDNAs. Recently, it has been demonstrated that musclin is involved in the pathogenesis of spontaneously hypertensive rats (SHRs). However, it is known as a genetic hypertension model. In the present study, we aim to investigate the role of musclin in another animal model of hypertension and characterize the direct effect of musclin on vascular contraction. The results show that expression of musclin was increased in arterial tissues isolated from DOCA-salt induced hypertensive rats or the normal rats received repeated vasoconstriction with phenylephrine. Additionally, direct incubation with phenylephrine did not modify the expression of musclin in the in vitro studies. Also, the direct effect of musclin on the increase of intracellular calcium was observed in a concentration-dependent manner. These results provide the evidence to support that musclin is involved in hypertension. Thus, musclin is suitable to be considered as a novel target for treatment of hypertension.
肌肉素是从小鼠骨骼肌cDNA的信号序列陷阱中发现的一种新型骨骼肌衍生因子。最近，有研究表明肌肉素参与自发性高血压大鼠（SHR）的发病机制。然而，它是一种已知的遗传性高血压模型。在本研究中，我们旨在研究肌肉素在另一种高血压动物模型中的作用，并表征肌肉素对血管收缩的直接影响。结果表明，从去氧皮质酮盐诱导的高血压大鼠或接受去氧肾上腺素反复血管收缩的正常大鼠分离的动脉组织中，肌肉素的表达增加。此外，在体外研究中，与去氧肾上腺素直接孵育不会改变肌肉素的表达。而且，观察到肌肉素对细胞内钙增加的直接作用呈浓度依赖性。这些结果提供了支持肌肉素参与高血压的证据。因此，肌肉素适合被视为治疗高血压的新靶点。
