P物质免疫反应性在膈神经初级传入神经元中的定位。
Localization of substance P immunoreactivity in phrenic primary afferent neurons.
作者信息
Holtman J R
机构信息
Department of Pharmacology, College of Medicine, University of Kentucky, Lexington 40536.
出版信息
Peptides. 1989 Jan-Feb;10(1):53-6. doi: 10.1016/0196-9781(89)90075-2.
Retrograde tracing with a fluorescent dye (Fast Blue) combined with immunohistochemistry was used to localize the putative neurotransmitter, substance P, in phrenic primary afferent neurons. Fast Blue was injected into the diaphragm and was found to label phrenic primary afferent neurons in sections from the fifth and sixth cervical dorsal root ganglia. The same sections were then treated with antiserum to substance P. A total of 11.4% of labelled phrenic primary afferent neurons contained substance P immunoreactivity. The diameters of the neurons ranged between 17 to 45 microns with a mean size of 29.7 +/- 0.7 microns (N = 81). The results suggest that substance P could be involved in mediating the transmission of sensory information from the diaphragm to the CNS.
采用荧光染料（快蓝）逆行追踪结合免疫组织化学方法，在膈神经初级传入神经元中定位假定的神经递质P物质。将快蓝注入膈肌，发现在第五和第六颈背根神经节切片中标记了膈神经初级传入神经元。然后对相同切片用抗P物质血清处理。总共11.4%的标记膈神经初级传入神经元含有P物质免疫反应性。神经元直径在17至45微米之间，平均大小为29.7 +/- 0.7微米（N = 81）。结果表明，P物质可能参与介导从膈肌到中枢神经系统的感觉信息传递。
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