Shao Wanyue, Sun Zhengang, Hua Ruinian, Zhang Wei, Zhao Jun, Na Liyan
J Nanosci Nanotechnol. 2014 May;14(5):3887-92. doi: 10.1166/jnn.2014.7972.
Water-soluble BaYF5:Yb3+/Er3+ nanoparticles with the surface functionalized by a layer of poly(acrylic acid) (PAA) were synthesized via a facile one-step PAA-assisted hydrothermal method. Bovine serum albumin (BSA) protein was conjugated with BaYF5:Yb3+/Er3+ upconversion nanoparticles via free carboxylic acid groups on the surface of nanoparticles. The final products were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), infrared (IR) spectrophotometry, ultraviolet (UV) spectrophotometry and photoluminescence spectroscopy (PL). The XRD results showed that PAA-capped BaYF5:Yb3+/Er3+ upconversion nanoparticles could be obtained via a PAA assisted hydrothermal process with the pH value of 8 at 200 degrees C for 24 h. The TEM results showed that the morphology of BaYF5:Yb3+/Er3+ nanoparticles was spherical particles with an average diameter of about 4 nm. The IR and UV spectra showed that BSA has been conjugated with BaYF5:Yb3+/Er3+ up-conversion nanoparticles. The luminescence properties of BaYF5:Yb3+/Er3+ up-conversion nanoparticles were also studied. The luminescence properties of the products suggest that BaYF5:Yb3+/Er3+ upconversion nanoparticles have promising applications for labels in biological assays.
通过简便的一步法聚丙烯酸（PAA）辅助水热法合成了表面由一层聚丙烯酸（PAA）功能化的水溶性BaYF5:Yb3+/Er3+纳米颗粒。牛血清白蛋白（BSA）蛋白通过纳米颗粒表面的游离羧酸基团与BaYF5:Yb3+/Er3+上转换纳米颗粒共轭。最终产物通过X射线衍射（XRD）、透射电子显微镜（TEM）、红外（IR）分光光度法、紫外（UV）分光光度法和光致发光光谱（PL）进行表征。XRD结果表明，通过PAA辅助水热过程，在200℃、pH值为8的条件下反应24小时，可以获得PAA包覆的BaYF5:Yb3+/Er3+上转换纳米颗粒。TEM结果表明，BaYF5:Yb3+/Er3+纳米颗粒的形态为球形颗粒，平均直径约为4nm。IR和UV光谱表明BSA已与BaYF5:Yb3+/Er3+上转换纳米颗粒共轭。还研究了BaYF5:Yb3+/Er3+上转换纳米颗粒的发光特性。产物的发光特性表明BaYF5:Yb3+/Er3+上转换纳米颗粒在生物分析标记方面具有广阔的应用前景。
