Department of Microbial Natural Products, Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI) and Pharmaceutical Biotechnology, Saarland University , Campus C2 3, 66123 Saarbrücken, Germany.
Org Lett. 2014 May 2;16(9):2414-7. doi: 10.1021/ol500779s. Epub 2014 Apr 15.
Cystomanamides A-D were isolated as novel natural product scaffolds from Cystobacter fuscus MCy9118, and their structures were established by spectroscopic techniques including 2D NMR, LC-SPE-NMR/-MS, and HR-MS. The cystomanamides contain β-hydroxy amino acids along with 3-amino-9-methyldecanoic acid that is N-glycosylated in cystomanamide C and D. The gene cluster for cystomanamide biosynthesis was identified by gene disruption as PKS/NRPS hybrid incorporating an iso-fatty acid as starter unit and including a reductive amination step at the interface of the PKS and NRPS modules.
从粘质沙雷氏菌 MCy9118 中分离到了四种新型天然产物支架的囊泡霉素 A-D，并通过包括 2D NMR、LC-SPE-NMR/-MS 和 HR-MS 在内的光谱技术确定了它们的结构。这些囊泡霉素含有β-羟基氨基酸和 N-糖基化的 3-氨基-9-甲基癸酸，存在于囊泡霉素 C 和 D 中。通过基因敲除鉴定了囊泡霉素生物合成的基因簇，该基因簇为 PKS/NRPS 杂合子，采用异脂肪酸作为起始单元，并在 PKS 和 NRPS 模块的界面处包含还原胺化步骤。
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