使用单克隆抗体通过酶联免疫吸附测定法测定人粒细胞集落刺激因子
Measurement of human G-CSF by enzyme-linked immunosorbent assay using monoclonal antibody.
作者信息
Omori F, Okamura S, Takaku F, Niho Y
机构信息
Cancer Center, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
出版信息
Res Exp Med (Berl). 1989;189(3):163-71. doi: 10.1007/BF01852164.
An IgG monoclonal antibody to recombinant human granulocyte colony-stimulating factor (G-CSF), designated HG1, was produced by fusion of immune mouse splenocytes with HAT-sensitive murine myeloma cells. This HG1 was capable of neutralizing the colony-stimulating activity of G-CSF in vitro, and it did not cross-react with human granulocyte/macrophage colony-stimulating factor (GM-CSF). An enzyme-linked immunosorbent assay (ELISA) for measurement of G-CSF was developed using HG1 and a polyclonal antibody against G-CSF raised in a rabbit. The data indicated that the ELISA was highly efficient and sensitive for the detection of as little as 50 pg/ml of recombinant G-CSF. This assay system therefore warrants further attention.
一种针对重组人粒细胞集落刺激因子（G-CSF）的IgG单克隆抗体，命名为HG1，是通过将免疫小鼠脾细胞与对氨基蝶呤-氨基喋呤-胸腺嘧啶核苷（HAT）敏感的小鼠骨髓瘤细胞融合产生的。这种HG1能够在体外中和G-CSF的集落刺激活性，并且它与人粒细胞/巨噬细胞集落刺激因子（GM-CSF）不发生交叉反应。使用HG1和在兔体内产生的针对G-CSF的多克隆抗体制备了一种用于测量G-CSF的酶联免疫吸附测定（ELISA）。数据表明，该ELISA对于检测低至50 pg/ml的重组G-CSF具有高效性和敏感性。因此，该检测系统值得进一步关注。
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