Alieva I B, Vorob'ev I A
Tsitologiia. 1989 Mar;31(3):259-66.
Calcium ionophore A23187, taken at a concentration of 0.1 microgram/ml, quickly uncouples mitochondria in PE culture cells in medium 199. The cell ultrastructure undergoes reversible changes (especially that of mitochondria): maximum changes occur 2 hours after the start of the treatment; in 8 hours they become less pronounced. The adaptation of cells does not involve the ionophore inactivation in the medium. 10 micrograms/ml of A23187 induces gradual but irreversible alterations. Microtubules in PE cells are not destroyed when incubated in medium 199 containing 10 micrograms/ml of A23187 and 11 mM Ca2+. The addition of 10 micrograms/ml ionophore to the normal 199 medium (1.26 mM Ca2+) results in the formation of electron dense bodies in the cell center 30 minutes after the start of incubation. These bodies disappear in the course of a subsequent incubation. The number of cells with primary cilia decreases. The percentage of centrioles located perpendicularly to the substrate increases 30 minutes following treatment with 0.1 microgram/ml A23187 in medium 199. 2 hours after the start of treatment with 0.1 microgram/ml ionophore no such changes are detected; an electron dense halo appears around the centriolar cylinders. 8 hours after the start of treatment the structure of the cell center does not differ from the normal one.
钙离子载体A23187在浓度为0.1微克/毫升时，能迅速使199培养基中的PE培养细胞中的线粒体解偶联。细胞超微结构发生可逆变化（尤其是线粒体的超微结构）：处理开始后2小时变化最大；8小时后变化变得不那么明显。细胞的适应性变化并不涉及培养基中离子载体的失活。10微克/毫升的A23187会诱导逐渐但不可逆的改变。当在含有10微克/毫升A23187和11毫摩尔钙离子的199培养基中孵育时，PE细胞中的微管不会被破坏。向正常的199培养基（1.26毫摩尔钙离子）中添加10微克/毫升离子载体，孵育开始30分钟后，细胞中心会形成电子致密体。在随后的孵育过程中，这些体会消失。具有初级纤毛的细胞数量减少。用0.1微克/毫升A23187在199培养基中处理30分钟后，垂直于底物定位的中心粒百分比增加。用0.1微克/毫升离子载体处理开始2小时后，未检测到此类变化；中心粒圆柱体周围出现电子致密晕。处理开始8小时后，细胞中心的结构与正常结构无异。
