Wounding stress causes rapid increase in concentration of the naturally occurring 2',3'-isomers of cyclic guanosine- and cyclic adenosine monophosphate (cGMP and cAMP) in plant tissues.

3',5'-Cyclic guanosine monophosphate (cGMP) and 3',5'-cyclic adenosine monophosphate (cAMP) are well reported second messenger molecules involved in cellular signal transduction, in physiological functions such as neurotransmission in animals and in the modulation of cell growth and differentiation. In plants, 3',5'-cyclic nucleotides have been implicated in the regulation of ion homeostasis, hormone and stress responses. The behavior of the 2',3'-cyclic nucleotide variants is also known in animal tissue but no quantitative information is available about 2',3'-cAMP and 2',3'-cGMP in plant material. A recently developed HILIC-SPE/LC-MS/MS method for the analysis of cyclic nucleotides in blood and animal tissue was therefore adapted to measure 2',3'-cAMP and 2',3'-cGMP concentrations in plant material. Cyclic nucleotide concentrations were measured in Arabidopsis thaliana (Col-0) leaves before and after the application of wounding stress. A significant (∼5-fold) up-regulation of 2',3'-cAMP and 2',3'-cGMP was measured in Arabidopsis leaves compared to the control samples. The results indicate a thus far unreported strong correlation between plant stress and both 2',3'-cAMP and 2',3'-cGMP levels in plant material, and may open new avenues towards understanding the role of cyclic nucleotides in plants.