Yam L T
Special Hematology Laboratory, Veterans Administration Medical Center, Louisville, KY 40202.
Acta Cytol. 1989 Jul-Aug;33(4):505-10.
Immunocytochemical studies were performed on fine needle aspirates of the liver in a patient with hepatocellular carcinoma. A panel of commercially available antibodies was used to study the aspirated cells by immunoalkaline phosphatase and immunoperoxidase methods. The malignant cells in the aspirates, which were positively stained by the immunoperoxidase method for alphafetoprotein and by both methods for epithelial membrane antigen, were most probably hepatocellular in origin. Some cells were shown by the immunoalkaline phosphatase method to possess leukocyte-common antigen (LCA) and antigens of colonic and ovarian tissues. These findings were further investigated, and it was found that the tumor cells indeed had LCA as well as levamisole-resistant alkaline phosphatase activity. Although the immunoalkaline phosphatase methods are useful immunodiagnostic techniques applicable to fine needle aspirates, the endogenous enzyme activity present in some nonhematopoietic tumor cells is a cause for caution in the use of these methods in aspirates from nonhematopoietic tumor tissues.
对一名肝细胞癌患者的肝脏细针穿刺抽吸物进行了免疫细胞化学研究。使用一组市售抗体，通过免疫碱性磷酸酶和免疫过氧化物酶方法对抽吸细胞进行研究。抽吸物中的恶性细胞，通过免疫过氧化物酶法对甲胎蛋白呈阳性染色，通过两种方法对上皮膜抗原呈阳性染色，最可能起源于肝细胞。免疫碱性磷酸酶方法显示一些细胞具有白细胞共同抗原（LCA）以及结肠和卵巢组织的抗原。对这些发现进行了进一步研究，发现肿瘤细胞确实具有LCA以及左旋咪唑抗性碱性磷酸酶活性。尽管免疫碱性磷酸酶方法是适用于细针穿刺抽吸物的有用免疫诊断技术，但一些非造血肿瘤细胞中存在的内源性酶活性是在使用这些方法处理非造血肿瘤组织的抽吸物时需要谨慎的原因。
