Vila Nova M X, Leite N G A, Houllou L M, Medeiros L V, Lira Neto A C, Hsie B S, Borges-Paluch L R, Santos B S, Araujo C S F, Rocha A A, Costa A F
Department of Neurosurgery, Spine and Spinal Cord Institute, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea
Department of Neurosurgery, Spine and Spinal Cord Institute, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
Genet Mol Res. 2014 Mar 31;13(1):2323-32. doi: 10.4238/2014.March.31.12.
The cowpea weevil (Callosobruchus maculatus Fabr.) is the most destructive pest of the cowpea bean; it reduces seed quality. To control this pest, resistance testing combined with genetic analysis using molecular markers has been widely applied in research. Among the markers that show reliable results, the inter-simple sequence repeats (ISSRs) (microsatellites) are noteworthy. This study was performed to evaluate the resistance of 27 cultivars of cowpea bean to cowpea weevil. We tested the resistance related to the genetic variability of these cultivars using ISSR markers. To analyze the resistance of cultivars to weevil, a completely randomized test design with 4 replicates and 27 treatments was adopted. Five pairs of the insect were placed in 30 grains per replicate. Analysis of variance showed that the number of eggs and emerged insects were significantly different in the treatments, and the means were compared by statistical tests. The analysis of the large genetic variability in all cultivars resulted in the formation of different groups. The test of resistance showed that the cultivar Inhuma was the most sensitive to both number of eggs and number of emerged adults, while the TE96-290-12-G and MNC99-537-F4 (BRS Tumucumaque) cultivars were the least sensitive to the number of eggs and the number of emerged insects, respectively.
豇豆象（Callosobruchus maculatus Fabr.）是豇豆最具破坏性的害虫，它会降低种子质量。为了防治这种害虫，将抗性测试与使用分子标记的遗传分析相结合已在研究中广泛应用。在显示可靠结果的标记中，简单序列重复区间（ISSRs）（微卫星）值得关注。本研究旨在评估27个豇豆品种对豇豆象的抗性。我们使用ISSR标记测试了这些品种与遗传变异性相关的抗性。为了分析品种对豆象的抗性，采用了完全随机试验设计，有4次重复和27个处理。每个重复中，将5对昆虫放置在30粒种子中。方差分析表明，各处理中卵数和羽化昆虫数存在显著差异，并通过统计检验比较均值。对所有品种的大遗传变异性分析导致形成了不同的组。抗性测试表明，Inhuma品种对卵数和羽化成虫数最为敏感，而TE96 - 290 - 12 - G和MNC99 - 537 - F4（BRS Tumucumaque）品种分别对卵数和羽化昆虫数最不敏感。
