Caslavska Jitka, Joneli Jeannine, Wanzenried Ursula, Schiess Jeannette, Lanz Christian, Thormann Wolfgang
Clinical Pharmacology Laboratory, Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
J Sep Sci. 2014 Jul;37(13):1663-70. doi: 10.1002/jssc.201400243. Epub 2014 May 16.
High-resolution capillary zone electrophoresis in the routine arena with stringent quality assurance is employed for the determination of carbohydrate-deficient transferrin in human serum. The assay comprises mixing of human serum with a Fe(III) -containing solution prior to analysis of the iron-saturated mixture in a dynamically double-coated capillary using a commercial buffer at alkaline pH. In contrast to other assays, it provides sufficient resolution for proper recognition of genetic transferrin variants. Analysis of 7290 patient sera revealed 166 isoform patterns that could be assigned to genetic variants, namely, 109 BC, 53 CD, one BD and three CC variants. Several subtypes of transferrin D can be distinguished as they have large enough differences in pI values. Subtypes of transferrin C and B cannot be resolved. However, analysis of the detection time ratios of tetrasialo isoforms of transferrin BC and transferrin CD variants revealed multimodal frequency histograms, indicating the presence of subtypes of transferrin C, B and D. The data gathered over 11 years demonstrate the robustness of the high-resolution capillary zone electrophoresis assay. This is the first account of a capillary zone electrophoresis based carbohydrate-deficient transferrin assay with a broad overview on transferrin isoform patterns associated with genetic transferrin variants.
在严格质量保证的常规环境中，高分辨率毛细管区带电泳用于测定人血清中缺乏碳水化合物的转铁蛋白。该测定法包括在使用碱性pH的商业缓冲液在动态双涂层毛细管中分析铁饱和混合物之前，将人血清与含铁（III）溶液混合。与其他测定法相比，它为正确识别遗传转铁蛋白变体提供了足够的分辨率。对7290份患者血清的分析揭示了166种可归因于遗传变体的同工型模式，即109种BC、53种CD、1种BD和3种CC变体。转铁蛋白D的几种亚型可以区分，因为它们的pI值有足够大的差异。转铁蛋白C和B的亚型无法分辨。然而，对转铁蛋白BC和转铁蛋白CD变体的四唾液酸同工型的检测时间比的分析揭示了多峰频率直方图，表明存在转铁蛋白C、B和D的亚型。11年来收集的数据证明了高分辨率毛细管区带电泳测定法的稳健性。这是首次对基于毛细管区带电泳的缺乏碳水化合物的转铁蛋白测定法进行报道，并对与遗传转铁蛋白变体相关的转铁蛋白同工型模式进行了广泛概述。
