Haghgoo R, Niyakan M, Nazari Moghaddam K, Asgary S, Mostafaloo N
Dept. of Pediatric Dentistry, Dental School, Shahed University, Tehran, Iran.
Dept. of Microbiology, Medical School, Shahed University, Tehran, Iran.
J Dent (Shiraz). 2014 Mar;15(1):28-32.
One of the most challenging procedural accidents during pulpotomy of primary molars is furcal perforation. To prevent bacterial invasion, the perforation site should be sealed as soon as possible.
The aim of the current study is to investigate the ability of the pro-root MTA and new endodontic cement (NEC) in repairing the furcation perforations of primary molar teeth.
In this in vitro study, 42 extracted primary molars were selected. Their roots were sectioned horizontally and standard access cavity was prepared. The orifices and the root apices were sealed with two layers of resin composite.The samples were randomly assigned into 2 groups. 6 teeth were considered as the positive and the negative controls. In the experimental groups; perforation was made. In group 1 and 2, perforation site received pro-root MTA and NEC respectively. The teeth were covered by two layers of nail polish except for the external surface of the perforation site. The negative control group received no repairing material. All teeth were mounted and sterilized for 24 hours. Lower chambers were filled with sterilized Muller Hinton broth. Bacterial suspension of Enterococcus faecalis in 0.5 McFarland was prepared. The repaired site was then exposed to the bacterial suspension of Enterococcus faecalis every 3 days. All samples were inserted in an incubator at 37(o)C and 100% humidity. The turbidity of the samples was detected for a period of 30 days. Data were analyzed by Chi- square test.
44% of samples in Pro- root group, 50% of the samples in the NEC group showed contaminations during 30 days. There was no significant difference between these two groups (p= 0.799).
With limitations of this study, Pro- root MTA and NEC showed similar capability in sealing the furcal perforations of the primary molars.
乳牙活髓切断术中最具挑战性的手术意外之一是髓室底穿孔。为防止细菌侵入，穿孔部位应尽快封闭。
本研究旨在探讨预成根MTA和新型牙髓黏固剂（NEC）修复乳牙髓室底穿孔的能力。
在这项体外研究中，选取了42颗拔除的乳牙。将其牙根水平切断并制备标准的开髓腔。髓室口和根尖用两层树脂复合体封闭。样本随机分为2组。6颗牙齿作为阳性和阴性对照。在实验组中制造穿孔。在第1组和第2组中，穿孔部位分别使用预成根MTA和NEC。除穿孔部位的外表面外，牙齿用两层指甲油覆盖。阴性对照组不使用修复材料。所有牙齿进行固定并消毒24小时。下腔室填充灭菌的穆勒-欣顿肉汤。制备0.5麦氏浊度的粪肠球菌细菌悬液。然后每隔3天将修复部位暴露于粪肠球菌细菌悬液中。所有样本置于37℃、湿度100%的培养箱中。检测样本的浊度，持续30天。数据采用卡方检验进行分析。
预成根组44%的样本、NEC组50%的样本在30天内出现污染。这两组之间无显著差异（p = 0.799）。
鉴于本研究的局限性，预成根MTA和NEC在封闭乳牙髓室底穿孔方面显示出相似的能力。
