Gu Peng, Zhou Yi-Bin, Yang Dong-Rong, Shan Yu-Xi, Xue Bo-Xin
Zhonghua Nan Ke Xue. 2014 Mar;20(3):225-8.
To explore the effects of stromal interaction molecule 1 (STIM1) on the expression of apoptosis-related proteins in prostate cancer PC-3 cells.
We transfected the lentivirus vector STIM1-pGCSIL-GFP carrying STIM shRNA into human hormone-independent prostate cancer PC-3 cells, and 3 days later observed the transfection efficiency by fluorescence microscopy. At 7 days after transfection, we determined the expression of STIM1 in the PC-3 cells by RT-PCR and Western blot and those of apoptosis-related proteins Bcl-2, Bax, survivin and activated Caspase-3 by Western blot.
At 3 days, inverted microscopy revealed a transfection efficiency of > 80%. At 7 days, the STIM1 expression was significantly inhibited at both mRNA and protein levels. The Bcl-2/Bax rate was remarkably decreased as compared with that of the control group (0. 31 vs 1.24 ) , and the survivin expression was markedly reduced, 0. 14 times that of the relative expression in the control. However, the Caspase-3 cleavage was significantly activated, 1.52 times that of the control (P <0.05).
STIM1 can be regarded as an oncogene in prostate cancer PC-3 cells. Inhibition of its expression can induce PC-3 cell apoptosis by reducing the Bcl-2/Bax rate, decreasing the survivin expression, and activating the Caspase-3 pathway.
探讨基质相互作用分子1（STIM1）对前列腺癌PC-3细胞凋亡相关蛋白表达的影响。
将携带STIM shRNA的慢病毒载体STIM1-pGCSIL-GFP转染至人激素非依赖性前列腺癌PC-3细胞，3天后通过荧光显微镜观察转染效率。转染后7天，通过RT-PCR和Western blot检测PC-3细胞中STIM1的表达，通过Western blot检测凋亡相关蛋白Bcl-2、Bax、survivin和活化的Caspase-3的表达。
3天时，倒置显微镜显示转染效率>80%。7天时，STIM1在mRNA和蛋白水平均显著受到抑制。与对照组相比，Bcl-2/Bax比值显著降低（0.31对1.24），survivin表达明显减少，为对照组相对表达量的0.14倍。然而，Caspase-3的切割显著激活，为对照组的1.52倍（P<0.05）。
STIM1可被视为前列腺癌PC-3细胞中的癌基因。抑制其表达可通过降低Bcl-2/Bax比值、减少survivin表达和激活Caspase-3途径诱导PC-3细胞凋亡。
