Mittal Seema, Sharma Madhu, Chaudhary Uma
Department of Microbiology, Pt. B.D. Sharm PGIMS, Rohtak, Haryana, India.
Indian J Pathol Microbiol. 2014 Jan-Mar;57(1):61-4. doi: 10.4103/0377-4929.130899.
Urinary tract infection (UTI) is one of the most common nosocomial infections, caused by Escherichia coli. This study determined the presence of virulence factors in the organism and correlates it with the multi-drug resistance (MDR).
The aim of the following study is to assess the virulence factors of uropathogenic E. coli and antibiotic susceptibility pattern.
This was a prospective study conducted in the Department of Microbiology in PT. B. D. Sharma, PGIMS, Rohtak.
The study was conducted over a period of 1 year. Urine samples received were processed as per standard microbiological procedures. Virulence factors such as hemolysin, hemagglutination, cell surface hydrophobicity, serum resistance, gelatinase and siderophore production were studied. The antimicrobial susceptibility was done as per Clinical and Laboratory Standard Institute Guidelines.
The data was analyzed by using SPSS(Statistical Package for the social sciences) IBM Corporation version 17.0. A two sided P ≤ 0.05 was considered to be significant.
Hemolysin production was seen in 47.4%, hemagglutination in 74.8%, cell surface hydrophobicity in 61%, serum resistance in 59%, gelatinase in 67.5% and siderophore production in 88% isolates. Nitrofurantoin was found to be most effective followed by, gatifloxacin and gentamicin. Twenty nine percent (29.62%) isolates were MDR.
Therefore, the knowledge of virulence factors of E. coli and their antibiotic susceptibility pattern will help in better understanding of the organism and in the treatment of UTI.
尿路感染（UTI）是由大肠杆菌引起的最常见的医院感染之一。本研究确定了该菌中致病因子的存在，并将其与多重耐药性（MDR）相关联。
以下研究的目的是评估尿路致病性大肠杆菌的致病因子和抗生素敏感性模式。
这是一项在罗塔克市PGIMS的PT. B. D. 夏尔马微生物学系进行的前瞻性研究。
该研究持续了1年。所接收的尿液样本按照标准微生物学程序进行处理。研究了溶血素、血凝、细胞表面疏水性、血清抗性、明胶酶和铁载体产生等致病因子。根据临床和实验室标准协会指南进行抗菌药物敏感性检测。
数据使用IBM公司的SPSS（社会科学统计软件包）版本17.0进行分析。双侧P≤0.05被认为具有统计学意义。
47.4%的分离株产生溶血素，74.8%产生血凝，61%具有细胞表面疏水性，59%具有血清抗性，67.5%产生明胶酶，88%产生铁载体。发现呋喃妥因最有效，其次是加替沙星和庆大霉素。29.62%的分离株为多重耐药。
因此，了解大肠杆菌的致病因子及其抗生素敏感性模式将有助于更好地了解该菌并治疗尿路感染。
