Talwar H S, Fisher G J, Harris V A, Voorhees J J
Department of Dermatology, University of Michigan Medical School, Ann Arbor 48109.
J Invest Dermatol. 1989 Aug;93(2):241-5. doi: 10.1111/1523-1747.ep12277581.
The ability of a variety of agonists to induce formation of inositol phosphates and 1,2-diacylglycerol in cultured adult human keratinocytes has been investigated. Histamine, bradykinin, and thrombin significantly stimulated formation of inositol mono-, bis-, and trisphosphate and 1,2-diacylglycerol within 5 min after addition. Aluminum fluoride also caused a dose-dependent accumulation of inositol phosphates suggesting the participation of a GTP binding protein in the regulation of phospholipase C-catalyzed phosphoinositide hydrolysis. These data demonstrate that human keratinocytes possess the capacity for phospholipase C-mediated signal transduction and suggest that this pathway may participate in the regulation of keratinocyte function.
研究了多种激动剂在培养的成人人类角质形成细胞中诱导肌醇磷酸酯和1,2 - 二酰基甘油形成的能力。组胺、缓激肽和凝血酶在添加后5分钟内显著刺激了肌醇一磷酸、二磷酸、三磷酸以及1,2 - 二酰基甘油的形成。氟化铝也导致了肌醇磷酸酯的剂量依赖性积累，这表明一种GTP结合蛋白参与了磷脂酶C催化的磷酸肌醇水解的调节。这些数据表明人类角质形成细胞具有磷脂酶C介导的信号转导能力，并提示该途径可能参与角质形成细胞功能的调节。
