Wetzel D M, Lee V M, Erulkar S D
Section of Neurobiology and Behavior, Cornell University, Ithaca, New York 14853.
J Neurobiol. 1989 Jun;20(4):255-70. doi: 10.1002/neu.480200407.
Cells from adult Xenopus laevis brainstem and spinal cord were dissociated with mild enzymatic treatment and grown in long-term cell culture. These cells had specific attachment/substrate and medium/serum requirements. Cells with bipolar and multipolar morphology were positively identified as neurons using immunohistochemistry with monoclonal antibodies to rat and bovine neurofilament proteins which we show here cross-react with similar amphibian proteins. Patch clamp recordings demonstrated that these neurons have populations of ionic channels which are activated by L-glutamate or gamma-aminobutyric acid (GABA). The characteristics of these channels were similar to those previously described for GABA- and glutamate-activated channels in embryonic mammalian neurons isolated in culture. Cell cultures of neurons isolated from adult Xenopus laevis brain may be a useful and simple preparation with which to examine the modulation of neuronal properties by various agents over longer time intervals then has been previously possible.
成年非洲爪蟾脑干和脊髓的细胞经轻度酶处理后解离，并在长期细胞培养中生长。这些细胞有特定的附着/底物以及培养基/血清需求。使用针对大鼠和牛神经丝蛋白的单克隆抗体进行免疫组织化学鉴定，具有双极和多极形态的细胞被确认为神经元，我们在此表明这些抗体与类似的两栖动物蛋白发生交叉反应。膜片钳记录表明，这些神经元具有被L-谷氨酸或γ-氨基丁酸（GABA）激活的离子通道群体。这些通道的特性与先前在培养中分离的胚胎哺乳动物神经元中描述的GABA和谷氨酸激活通道的特性相似。从成年非洲爪蟾脑中分离的神经元细胞培养物可能是一种有用且简单的制备方法，通过它可以在比以前更长的时间间隔内研究各种试剂对神经元特性的调节。
