Cubbage M L, Suwanichkul A, Powell D R
Department of Pediatrics, Baylor College of Medicine, Diabetes Research Center, Houston, Texas 77054.
Mol Endocrinol. 1989 May;3(5):846-51. doi: 10.1210/mend-3-5-846.
We have characterized the structure of the human chromosomal gene for the 25 kilodalton insulin-like growth factor binding protein (BP-25) as a first step toward understanding both the factors which regulate BP-25 transcription and also the evolution of the insulin-like growth factor binding proteins. The BP-25 gene is present in the human genome as a single copy which spans 5.2 kilobases and contains four exons. Primer extension localizes the mRNA cap site 165 base pairs (bp) upstream of the ATG translational start codon. Preliminary analysis of the putative promoter region for BP-25 demonstrates characteristics consistent with those of many eukaryotic promoters; these include a consensus TATA box beginning 28 bp 5' to the cap site and a consensus CCAAT promoter element beginning 72 bp upstream from the cap site.
我们已经对25千道尔顿胰岛素样生长因子结合蛋白（BP - 25）的人类染色体基因结构进行了表征，这是了解调节BP - 25转录的因素以及胰岛素样生长因子结合蛋白进化的第一步。BP - 25基因在人类基因组中以单拷贝形式存在，跨度为5.2千碱基，包含四个外显子。引物延伸将mRNA帽位点定位在ATG翻译起始密码子上游165个碱基对（bp）处。对BP - 25假定启动子区域的初步分析表明其具有与许多真核启动子一致的特征；这些特征包括一个位于帽位点5'端28 bp处的共有TATA盒和一个位于帽位点上游72 bp处的共有CCAAT启动子元件。
