Rabinovici R, Gross D, Krausz M M
Department of Surgery B, Hadassah University Hospital, Jerusalem, Israel.
Surg Gynecol Obstet. 1989 Aug;169(2):137-42.
The effect of a solution of 7.5 per cent sodium chloride in 6.0 percent dextran 70 was studied in a rat model of uncontrolled hemorrhagic shock induced by partial resection of the tail of the rat. Fifty rats were randomly assorted into five groups of ten rats each. In group 1, uncontrolled hemorrhagic shock was induced by sharp resection of 10 per cent of the terminal portion of the tail of the rat. In group 2, uncontrolled hemorrhagic shock was treated with 5 milliliters per kilogram of 0.9 per cent sodium chloride. In group 3, uncontrolled hemorrhagic shock was treated with 5 milliliters per kilogram of 6 per cent dextran 70. In group 4, rats were treated with 5 milliliters per kilogram of 7.5 per cent sodium chloride, and in group 5, 5 milliliters per kilogram of 7.5 per cent sodium chloride in 6.0 per cent dextran 70 were infused. During the first five minutes, bleeding in rats in group 1 was 3.0 +/- 0.4 milliliters and the mean arterial pressure fell from 105 +/- 5 to 73 +/- 7 torr (p less than 0.001). In group 4, bleeding at 15, 30 and 60 minutes was 1.9 +/- 0.3 milliliters (p less than 0.02), 2.0 +/- 0.7 milliliters (p less than 0.04) and 2.4 +/- 1.1 milliliters (p less than 0.02), and in group 5, 2.5 +/- 0.6 milliliters (p less than 0.02), 2.2 +/- 0.6 milliliters (p less than 0.04) and 3.1 +/- 0.8 milliliters (p less than 0.04), respectively, compared with bleeding in group 1. Increased bleeding was followed by a fall in mean arterial pressure in groups 4 and 5 with a 60 per cent mortality rate (p less than 0.01) in both groups and respective, mean survival times of 135 +/- 29 and 144 +/- 26 minutes, which were significantly increased compared with group 1 (p less than 0.01 for both). The intravenous infusion of 6 per cent dextran 70 alone in group 3 resulted in delayed bleeding of 1.23 +/- 0.6 milliliters (p less than 0.04) after 180 minutes. It is concluded that infusions of small volume of 7.5 per cent sodium chloride or 7.5 per cent sodium chloride in 6.0 per cent dextran 70 solutions in rats in a state of uncontrolled hemorrhagic shock led to increased bleeding, decrease in mean arterial pressure and increased mortality.
在大鼠尾部部分切除诱导的失血性休克未控制模型中，研究了7.5%氯化钠与6.0%右旋糖酐70混合溶液的作用。50只大鼠被随机分为5组，每组10只。第1组，通过锐性切除大鼠尾部末端10%诱导失血性休克未控制。第2组，用每千克5毫升的0.9%氯化钠治疗失血性休克未控制。第3组，用每千克5毫升的6%右旋糖酐70治疗失血性休克未控制。第4组，大鼠用每千克5毫升的7.5%氯化钠治疗，第5组，输注每千克5毫升的7.5%氯化钠与6.0%右旋糖酐70混合溶液。在最初5分钟内，第1组大鼠的出血量为3.0±0.4毫升，平均动脉压从105±5降至73±7托（p<0.001）。第4组在15、30和60分钟时的出血量分别为1.9±0.3毫升（p<0.02）、2.0±0.7毫升（p<0.04）和2.4±1.1毫升（p<0.02），第5组分别为2.5±0.6毫升（p<0.02）、2.2±0.6毫升（p<0.04）和3.1±0.8毫升（p<0.04），与第1组相比。第4组和第5组出血量增加后平均动脉压下降，两组死亡率均为60%（p<0.01），平均生存时间分别为135±29分钟和144±26分钟，与第1组相比显著增加（两组均p<0.01）。第3组单独静脉输注6%右旋糖酐70在180分钟后导致延迟出血1.23±0.6毫升（p<0.04）。结论是，在失血性休克未控制状态的大鼠中输注小剂量的7.5%氯化钠或7.5%氯化钠与6.0%右旋糖酐70混合溶液会导致出血增加、平均动脉压降低和死亡率增加。
