Gregor-Mendel-Straße 4, 85350 Freising, Germany.
Gregor-Mendel-Straße 4, 85350 Freising, Germany.
Fungal Biol. 2014 Apr;118(4):385-93. doi: 10.1016/j.funbio.2014.02.003. Epub 2014 Mar 1.
Hydrophobins are small secreted proteins ubiquitously found in filamentous fungi. Some hydrophobins were shown to have functions in fungal development, while others lack known function. Class II hydrophobins from Fusarium graminearum and Fusarium culmorum are characterized by formation of low stability aggregates and their solubility in organic solvents. They are economically relevant to the brewing industry because they can induce beer gushing. Since cellular functions of Hyd5p's are still unknown, we analyzed the influence of FgHyd5p on growth and morphology of F. graminearum using FgΔhyd5 knock-out mutants expressing sGFP under the control of the hyd5 promoter and compared them with the performance of the parent wild type strain. Results demonstrate that FgHyd5p does not affect the colony and hyphal morphology. FgHyd5p affects the hydrophobicity of aerial mycelia but had no obvious function in penetration of hyphae through the water air interface. The hydrophobin affects the morphology of conidia, but not their fitness. Different sources of carbon and nitrogen as well as different pH have no effect on the expression of the hyd5 gene, which was demonstrated to be expressed upon growth of F. graminearum on hydrophobic surfaces.
水蛋白是广泛存在于丝状真菌中的小分泌蛋白。一些水蛋白被证明在真菌发育中有功能，而其他水蛋白则缺乏已知的功能。来自禾谷镰刀菌和串珠镰刀菌的 II 类水蛋白的特点是形成低稳定性的聚集体，并且在有机溶剂中可溶。它们与酿造工业密切相关，因为它们可以引发啤酒喷涌。由于 Hyd5p 的细胞功能仍然未知，我们使用在 hyd5 启动子控制下表达 sGFP 的 FgΔhyd5 敲除突变体分析了 FgHyd5p 对禾谷镰刀菌生长和形态的影响，并将其与亲本野生型菌株的性能进行了比较。结果表明，FgHyd5p 不影响菌落和菌丝形态。FgHyd5p 影响气生菌丝的疏水性，但在菌丝穿透水-气界面方面没有明显作用。该水蛋白影响分生孢子的形态，但对其适应性没有影响。不同的碳源和氮源以及不同的 pH 值对 hyd5 基因的表达没有影响，该基因在禾谷镰刀菌生长在疏水性表面时被证明有表达。
