Translational Research Center for Gastrointestinal Disorders, Leuven, Belgium; and.
AstraZeneca R&D, Mölndal, Sweden.
Am J Physiol Gastrointest Liver Physiol. 2014 Jul 1;307(1):G122-8. doi: 10.1152/ajpgi.00043.2014. Epub 2014 Apr 17.
Pancreatic polypeptide (PP) is an anorexigenic hormone released from pancreatic F cells upon food intake. We aimed to determine the effect of PP on gastric accommodation and gastric emptying in conscious Wistar HAN rats to investigate whether effects on motor function could contribute to its anorexigenic effects. Intragastric pressure (IGP) was measured through a chronically implanted gastric fistula during the infusion of a nutrient meal (Nutridrink; 0.5 ml/min). Rats were treated with PP (0, 33 and 100 pmol·kg(-1)·min(-1)) in combination with N(G)-nitro-L-arginine methyl ester (L-NAME; 180 mg·kg(-1)·h(-1)), atropine (3 mg·kg(-1)·h(-1)), or vehicle. Furthermore, the effect of PP was tested after subdiaphragmal vagotomy of the stomach. Gastric emptying of a noncaloric and a caloric meal after treatment with 100 pmol·kg(-1)·min(-1) PP or vehicle was compared using X-rays. PP significantly increased IGP during nutrient infusion compared with vehicle (P < 0.01). L-NAME and atropine significantly increased IGP during nutrient infusion compared with vehicle treatment (P < 0.005 and 0.01, respectively). The effect of PP on IGP during nutrient infusion was abolished in the presence of L-NAME and in the presence of atropine. In vagotomized rats, PP increased IGP compared with intact controls (P < 0.05). PP significantly delayed gastric emptying of both a noncaloric (P < 0.05) and a caloric (P < 0.005) meal. PP inhibits gastric accommodation and delays gastric emptying, probably through inhibition of nitric oxide release. These results indicate that, besides the well-known centrally mediated effects, PP might decrease food intake through peripheral mechanisms.
胰多肽（PP）是一种摄食后由胰腺 F 细胞释放的厌食激素。我们旨在确定 PP 对清醒 Wistar HAN 大鼠胃容纳和胃排空的影响，以研究其对运动功能的影响是否有助于其厌食作用。通过慢性植入的胃瘘在营养餐（Nutridrink；0.5ml/min）输注期间测量胃内压（IGP）。用 PP（0、33 和 100pmol·kg(-1)·min(-1)）与 N(G)-硝基-L-精氨酸甲酯（L-NAME；180mg·kg(-1)·h(-1)）、阿托品（3mg·kg(-1)·h(-1)）或载体联合处理大鼠。此外，还在胃下膈迷走神经切断术后测试了 PP 的作用。用 100pmol·kg(-1)·min(-1) PP 或载体处理后，用 X 射线比较非热量和热量餐的胃排空。与载体相比，PP 在营养输注期间显着增加 IGP（P<0.01）。与载体相比，L-NAME 和阿托品在营养输注期间显着增加 IGP（P<0.005 和 0.01）。在存在 L-NAME 和存在阿托品的情况下，PP 对营养输注期间 IGP 的作用被消除。与完整对照相比，在迷走神经切断大鼠中，PP 增加了 IGP（P<0.05）。PP 显着延迟了非热量（P<0.05）和热量（P<0.005）餐的胃排空。PP 抑制胃容纳并延迟胃排空，可能通过抑制一氧化氮释放。这些结果表明，除了众所周知的中枢介导作用外，PP 还可能通过外周机制减少食物摄入。
