Yamamoto M, Gao F, Furuichi M, Ichiyoshi Y, Endo H
Department of Biochemistry, Kyushu University, Fukuoka, Japan.
Biochim Biophys Acta. 1989 Aug 14;1008(3):322-8. doi: 10.1016/0167-4781(89)90023-7.
The rat alpha 2u globulin gene family is composed of about 25 members with closely related structure. Several of these genes are transcriptionally active in the liver of adult males. We isolated about 50 independent genomic clones from the library and determined complete nucleotide sequences of two. Although they were alike and revealed structural features prerequisite for active genes, one cloned gene was interrupted in the 6th intron with about 900 bp 5' truncated and 3'-terminal triplicated form of LINE 1 sequence flanked by 16 bp complete direct repeat originating from the 6th intron. Within the inserted LINE 1 an excellent splicing acceptor homologue with a precedent lariat junction motif and multiple polyadenylation signals was identified. Thus, the possible production of a chimeric RNA, the last exon of which would be replaced by a part of LINE 1, has to be considered.
大鼠α2u球蛋白基因家族由约25个结构密切相关的成员组成。其中一些基因在成年雄性大鼠肝脏中具有转录活性。我们从文库中分离出约50个独立的基因组克隆，并测定了其中两个的完整核苷酸序列。尽管它们相似并揭示了活性基因必备的结构特征，但其中一个克隆基因在第6个内含子处中断，约900 bp的5'端截短，3'端为LINE 1序列的三倍体形式，两侧是源自第6个内含子的16 bp完全正向重复序列。在插入的LINE 1内，鉴定出一个具有前体套索状连接基序和多个聚腺苷酸化信号的优秀剪接受体同源物。因此，必须考虑可能产生嵌合RNA的情况，其最后一个外显子将被LINE 1的一部分所取代。
