Cavelti-Weder Claudia, Li Weida, Weir Gordon C, Zhou Qiao
Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center Boston, Boston, MA, USA.
Methods Mol Biol. 2014;1150:247-62. doi: 10.1007/978-1-4939-0512-6_17.
Pancreatic exocrine cells can be directly converted to insulin(+) beta cells by adenoviral-mediated expression of three transcription factors Pdx1, Mafa, and Ngn3 in the adult mouse pancreas (Zhou et al., Nature 455(7213):627-632, 2008). This direct reprogramming approach offers a strategy to replenish beta-cell mass and may be further developed as a potential future treatment for diabetes. Here, we provide a detailed protocol for inducing exocrine to beta-cell reprogramming in mice. We also describe key analyses we routinely use to assess the phenotype and function of reprogrammed cells.
在成年小鼠胰腺中，通过腺病毒介导的三种转录因子Pdx1、Mafa和Ngn3的表达，胰腺外分泌细胞可直接转化为胰岛素阳性的β细胞（Zhou等人，《自然》455(7213):627 - 632，2008年）。这种直接重编程方法提供了一种补充β细胞数量的策略，并且可能进一步发展成为未来治疗糖尿病的潜在方法。在这里，我们提供了一份在小鼠中诱导外分泌细胞向β细胞重编程的详细方案。我们还描述了我们常规用于评估重编程细胞的表型和功能的关键分析方法。
