MG-63细胞在不同参数的胶原蛋白、聚-L-乳酸、羟基磷灰石支架上的生物相容性。
Biocompatibility of MG-63 cells on collagen, poly-L-lactic acid, hydroxyapatite scaffolds with different parameters.
作者信息
Cecen Berivan, Kozaci Didem, Yuksel Mithat, Erdemli Diler, Bagriyanik Alper, Havitcioglu Hasan
机构信息
1 Department of Biomechanics, The Institute of Health Science, Dokuz Eylul University, Izmir - Turkey.
出版信息
J Appl Biomater Funct Mater. 2015 Mar 18;13(1):10-6. doi: 10.5301/jabfm.5000182.
PURPOSE
In this study, osteoblast-like MG-63 cells were cultured on 3 different scaffold types composed of (a) collagen + poly-L-lactic acid (PLLA), (b) collagen + hydroxyapatite (HA; 30ºC) or (c) collagen + hydroxyapatite (HA; 37ºC) and produced with different porosities.
METHODS
Biomechanical properties of the scaffolds were characterized by tensile strength measurements. Properties of the cell-seeded scaffolds were evaluated with scanning electron microscopy (SEM). Cell adhesion and proliferation capacities were evaluated. Alkaline phosphatase (ALP) levels in media were measured. Transmission electron microscopy (TEM) and histological analyses were used to assess morphological characteristics.
RESULTS
Our results showed that collagen-based PLLA and HA scaffolds have good cell biocompatibility. MTT test showed that the scaffolds exhibited no cytotoxicity. According to the force and displacement data, collagen + HA at 37ºC showed the highest mechanical strength and displacement.
CONCLUSION
The results suggest that collagen-based PLLA and HA scaffolds might improve osteoblastic growth in vitro and have biomaterial integration potential in possible therapeutic approaches for future clinical studies.
目的
在本研究中，将成骨样MG-63细胞培养在由以下三种不同支架类型组成的材料上：（a）胶原蛋白+聚-L-乳酸（PLLA），（b）胶原蛋白+羟基磷灰石（HA；30℃）或（c）胶原蛋白+羟基磷灰石（HA；37℃），且这些材料具有不同的孔隙率。
方法
通过拉伸强度测量来表征支架的生物力学性能。用扫描电子显微镜（SEM）评估接种细胞的支架的性能。评估细胞黏附和增殖能力。测量培养基中的碱性磷酸酶（ALP）水平。使用透射电子显微镜（TEM）和组织学分析来评估形态特征。
结果
我们的结果表明，基于胶原蛋白的PLLA和HA支架具有良好的细胞生物相容性。MTT试验表明这些支架无细胞毒性。根据力和位移数据，37℃的胶原蛋白+HA显示出最高的机械强度和位移。
结论
结果表明，基于胶原蛋白的PLLA和HA支架可能会改善体外成骨细胞的生长，并且在未来临床研究的可能治疗方法中具有生物材料整合潜力。
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