Department of Radioisotope Diagnostics and Radiopharmaceuticals, Medical University of Silesia, Poland.
Department of Analytical Chemistry, Medical University of Silesia, Poland.
Biol Sport. 2013 Jun;30(2):85-90. doi: 10.5604/20831862.1044222. Epub 2013 Apr 11.
The aim of this study was to assess the plasma adrenaline and noradrenaline concentrations as well as whole blood β2-adrenoceptor gene (ADRB2) expression in young ice hockey players before and immediately after exercise in relation to performed work. Nineteen Youth National Team ice hockey players were subjected to the maximal incremental cycloergometer exercise. The test was done in the pre-competitive phase of training. Among many parameters the plasma adrenaline and noradrenaline concentrations and ADRB2 gene expression in peripheral blood mononuclear cells (PBMC) were determined before and after exercise. The average performed work was 3261.3 ± 558.3 J · kg(-1) and maximal oxygen consumption (VO2max) for all players was 53.85 ± 3.91 mL · kg(-1) min(-1). The geometric mean of the ADRB2 gene expression was statistically significantly different before and after exercise (P ≤ 0.05), while adrenaline and noradrenaline levels in plasma significantly increased after exercise. In the analysed group of athletes we found that initial level of plasma noradrenaline correlated with the performed work (r = - 0.55, P < 0.014) and normalized ADRB2 expression before the exercise correlated with the work done by them (r = 0.48, P<0.039). However, no statistically significant correlations were found between the plasma adrenaline or noradrenaline concentrations and ADRB2 gene expression in peripheral blood of the players. The performed work in the maximal incremental exercise test of regularly training young ice hockey players depends on the initial levels of noradrenaline in plasma and ADRB2 mRNA in PBMC.
本研究旨在评估青年国家冰球队员在递增负荷踏车运动前和运动即刻的血浆肾上腺素和去甲肾上腺素浓度以及外周血单个核细胞（PBMC）中β2-肾上腺素能受体基因（ADRB2）的表达情况，并与完成的功量相关。19 名青年国家冰球队员进行了最大递增踏车运动测试。该测试在训练的竞赛前阶段进行。在许多参数中，测定了运动前和运动后血浆肾上腺素和去甲肾上腺素浓度以及 PBMC 中 ADRB2 基因表达。所有运动员的平均功量为 3261.3 ± 558.3 J·kg-1，最大摄氧量（VO2max）为 53.85 ± 3.91 mL·kg-1·min-1。ADRB2 基因表达的几何平均值在运动前后有统计学显著差异（P ≤ 0.05），而血浆中肾上腺素和去甲肾上腺素水平在运动后显著升高。在分析的运动员组中，我们发现初始血浆去甲肾上腺素水平与功量相关（r = -0.55，P < 0.014），运动前的标准化 ADRB2 表达与他们完成的功量相关（r = 0.48，P < 0.039）。然而，在运动员外周血的血浆肾上腺素或去甲肾上腺素浓度与 ADRB2 基因表达之间未发现统计学显著相关性。定期训练的青年冰球运动员在最大递增运动测试中完成的功量取决于血浆去甲肾上腺素和 PBMC 中 ADRB2 mRNA 的初始水平。
