Broers J L, de Leij L, Rot M K, ter Haar A, Lane E B, Leigh I M, Wagenaar S S, Vooijs G P, Ramaekers F C
Department of Pathology, University Hospital Nijmegen, The Netherlands.
Differentiation. 1989 May;40(2):119-28. doi: 10.1111/j.1432-0436.1989.tb00821.x.
The expression patterns of intermediate filament proteins in fetal and normal or nonpathological adult human lung tissues are described using (chain-specific) monoclonal antibodies. In early stages of development (9-10 weeks and 25 weeks of gestation) only so-called simple cytokeratins such as cytokeratins 7 (minor amounts). 8, 18 and 19 are detected in bronchial epithelial cells. At later stages of development, the cytokeratin expression patterns become more complex. The number of bronchial cells positive for cytokeratin 7 increases, but basal cells in the bronchial epithelium remain negative. These latter cells show, however, expression of cytokeratin 14 in the third trimester of gestation. Developing alveolar epithelial cells express cytokeratins 7, 8, 18 and 19. In adult human bronchial epithelium cytokeratins 4 (varying amounts), 7, 8, 13 (minor amounts), 14, 18 and 19 can be detected, with the main expression of cytokeratins 7, 8, and 18 in columnar cells and the main expression of cytokeratin 14 in basal cells. Vimentin is detected in all mesenchymal tissues. In addition, fetal lung expresses vimentin in bronchial epithelium, however, to a lesser extent with increasing age, resulting in the expression of vimentin in only few scattered bronchial cells at birth. Also in adult bronchial epithelium the expression of vimentin is noticed in part of the basal and columnar epithelial cells. Desmin filaments, present in smooth muscle cells of the lung, appear to alter their protein structure with age. In early stages of development smooth muscle cells surrounding blood vessels are partly reactive with some cytokeratin antibodies and with a polyclonal desmin antibody. At week 9-10 and week 25 of gestation a monoclonal antibody to desmin, however, is not reactive with blood vessel smooth muscle cells but is only reactive with smooth muscle cells surrounding bronchi. With increasing age the reactivity of cytokeratin antibodies with smooth muscle cells in blood vessels decreases, while the reactivity with the monoclonal desmin antibody increases. Our results show that during differentiation profound changes in the intermediate filament expression patterns occur in the different cell types of the developing lung.
利用（链特异性）单克隆抗体描述了中间丝蛋白在胎儿及正常或非病理性成人肺组织中的表达模式。在发育早期（妊娠9 - 10周和25周），仅在支气管上皮细胞中检测到所谓的简单细胞角蛋白，如细胞角蛋白7（少量）、8、18和19。在发育后期，细胞角蛋白的表达模式变得更加复杂。细胞角蛋白7阳性的支气管细胞数量增加，但支气管上皮中的基底细胞仍为阴性。然而，这些后者细胞在妊娠晚期显示细胞角蛋白14的表达。发育中的肺泡上皮细胞表达细胞角蛋白7、8、18和19。在成人支气管上皮中可检测到细胞角蛋白4（含量不同）、7、8、13（少量）、14、18和19，细胞角蛋白7、8和18主要在柱状细胞中表达，细胞角蛋白14主要在基底细胞中表达。波形蛋白在所有间充质组织中均有检测到。此外，胎儿肺在支气管上皮中表达波形蛋白，然而，随着年龄增长表达程度降低，导致出生时仅在少数散在的支气管细胞中表达波形蛋白。在成人支气管上皮中，也可在部分基底和柱状上皮细胞中注意到波形蛋白的表达。存在于肺平滑肌细胞中的结蛋白丝似乎随年龄改变其蛋白质结构。在发育早期，围绕血管的平滑肌细胞部分与一些细胞角蛋白抗体和多克隆结蛋白抗体反应。然而，在妊娠9 - 10周和25周时，一种抗结蛋白单克隆抗体与血管平滑肌细胞无反应，仅与围绕支气管的平滑肌细胞反应。随着年龄增长，细胞角蛋白抗体与血管平滑肌细胞的反应性降低，而与抗结蛋白单克隆抗体的反应性增加。我们的结果表明，在发育过程中，发育中肺的不同细胞类型的中间丝表达模式发生了深刻变化。
