Hessler H J, Pufahl C, Christophers E
Department of Dermatology, University of Kiel, FRG.
Int Arch Allergy Appl Immunol. 1989;89(2-3):236-41. doi: 10.1159/000234952.
Histamine release from peripheral blood basophils challenged with C5a, f-met-peptides and calcium ionophore was studied in patients with cold urticaria before and after exposure to low environmental temperatures. Compared to healthy controls, stimulated mediator release before cold exposure was increased in 7 of 11 patients. When challenged by cold exposure mediator release from in vitro-stimulated basophils was decreased. This decrease was more pronounced after stimulation with receptor-mediated stimuli (e.g. C5a) as compared to receptor-unrelated stimuli, e.g. calcium ionophore. In 4 of 11 patients stimulated mediator release before cold exposure was moderately increased. Also after cold exposure only a weak decrease of stimulated histamine release was seen. Levels of activated complement components (C3a) before and after cold exposure failed to provide evidence for complement activation in vivo. Also the number of circulating basophils as well as their cellular histamine content remained normal after cold exposure. The results show that in these patients release of histamine is altered before and after cold exposure. These changes in basophil responsiveness are not due to complement activation in vivo.
在环境温度降低之前和之后，研究了冷性荨麻疹患者外周血嗜碱性粒细胞在受到C5a、f-甲硫氨酸肽和钙离子载体刺激时组胺的释放情况。与健康对照相比，11名患者中有7名在冷暴露前刺激介质释放增加。当受到冷暴露刺激时，体外刺激的嗜碱性粒细胞的介质释放减少。与受体无关的刺激（如钙离子载体）相比，受体介导的刺激（如C5a）刺激后这种减少更为明显。11名患者中有4名在冷暴露前刺激介质释放适度增加。冷暴露后，刺激的组胺释放也仅出现微弱下降。冷暴露前后活化补体成分（C3a）的水平未能提供体内补体激活的证据。冷暴露后循环嗜碱性粒细胞的数量及其细胞组胺含量也保持正常。结果表明，在这些患者中，冷暴露前后组胺的释放发生了改变。嗜碱性粒细胞反应性的这些变化并非由于体内补体激活所致。
