Bisseret F, Keith G, Rihn B, Amiri I, Werneburg B, Girardot R, Baldacini O, Green G, Nguyen V K, Monteil H
Institut de Bactériologie de la Faculté de Médecine, Strasbourg, France.
J Chromatogr. 1989 May 5;490(1):91-100. doi: 10.1016/s0378-4347(00)82764-4.
The cytotoxin, also named toxin B, was isolated from a toxigenic strain of Clostridium difficile, purified to homogeneity and partially characterized. The purification procedure included ultrafiltration followed by anion-exchange chromatography. We noticed that a non-specific nucleic material eluted with the protein during the purification. The presence of these nucleic acids appeared to be important for the toxic activity of the protein. Some characteristics of the cytotoxin were examined, especially the amino acid composition and the sequence of three tryptic fragments.
这种细胞毒素，也称为毒素B，是从艰难梭菌的产毒菌株中分离出来的，纯化至同质并进行了部分特性鉴定。纯化程序包括超滤，然后进行阴离子交换色谱。我们注意到在纯化过程中有非特异性核酸物质与蛋白质一起洗脱。这些核酸的存在似乎对蛋白质的毒性活性很重要。对细胞毒素的一些特性进行了研究，特别是氨基酸组成和三个胰蛋白酶片段的序列。
