Ibrahim Mohammed E A, Wahab M Farooq, Lucy Charles A
Department of Chemistry, University of Alberta, Gunning/Lemieux Chemistry Centre, Edmonton, Alberta T6G 2G2, Canada.
Department of Chemistry, University of Alberta, Gunning/Lemieux Chemistry Centre, Edmonton, Alberta T6G 2G2, Canada.
Anal Chim Acta. 2014 Apr 11;820:187-94. doi: 10.1016/j.aca.2014.02.034. Epub 2014 Feb 26.
Hydrophilic interaction liquid chromatography (HILIC) is a fast growing separation technique for hydrophilic and polar analytes. In this work, we combine the unique selectivity of carbon surfaces with the high efficiency of core-shell silica. First, 5 μm core-shell silica is electrostatically coated with 105 nm cationic latex bearing quaternary ammonium groups. Then 50 nm anionic carbon nanoparticles are anchored onto the surface of the latex coated core-shell silica particles to produce a hybrid carbon-silica phase. The hybrid phase shows different selectivity than ten previously classified HILIC column chemistries and 36 stationary phases. The hybrid HILIC phase has shape selectivity for positional isomeric pairs (phthalic/isophthalic and 1-naphthoic/2-naphthoic acids). Fast and high efficiency HILIC separations of biologically important carboxylates, phenols and pharmaceuticals are reported with efficiencies up to 85,000 plates m(-1). Reduced plate height of 1.9 (95,000 plates m(-1)) can be achieved. The hybrid phase is stable for at least 3 months of usage and storage under typical HILIC eluents.
亲水作用液相色谱法（HILIC）是一种针对亲水性和极性分析物快速发展的分离技术。在本研究中，我们将碳表面独特的选择性与核壳硅胶的高效性相结合。首先，用带有季铵基团的105纳米阳离子胶乳对5微米的核壳硅胶进行静电涂覆。然后将50纳米的阴离子碳纳米颗粒锚定在涂覆了胶乳的核壳硅胶颗粒表面，以制备一种碳 - 硅混合相。该混合相展现出与之前分类的十种HILIC柱化学组成和36种固定相不同的选择性。这种混合HILIC相对位置异构体对（邻苯二甲酸/间苯二甲酸以及1 - 萘甲酸/2 - 萘甲酸）具有形状选择性。报道了对生物重要的羧酸盐、酚类和药物进行快速高效的HILIC分离，分离效率高达85,000理论塔板数每米（m⁻¹）。可实现1.9的折合塔板高度（95,000理论塔板数每米）。在典型的HILIC洗脱液条件下使用和储存时，该混合相至少3个月保持稳定。
