Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
J Chromatogr A. 2014 May 23;1343:128-34. doi: 10.1016/j.chroma.2014.03.072. Epub 2014 Apr 4.
Simple devices for the adsorption and preconcentration of metal ions comprising various monolithic polymers have been prepared by in situ polymerization within the 5.5cm long and 5.6mm i.d. polypropylene syringes. Poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith was modified with ethylenediamine to obtain the chelating material. The poly(butyl methacrylate-co-ethylene dimethacrylate) and poly(lauryl methacrylate-co-ethylene dimethacrylate) monoliths were first photografted with glycidyl methacrylate prior to functionalization with ethylenediamine. Alternatively, other chelating functionalities including poly(ethylene imines) varying in molecular weight and shape (linear and branched) as well as lysozyme were also attached to the monolithic supports. We found that the poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith functionalized with ethylenediamine exhibited the best chelating properties characterized with rapid adsorption and a capacity of 111.2mg/g (537μmol/g) for Pb(2+), 38.1mg/g (649μmol/g) for Ni(2+), 69.9mg/g (1100μmol/g) for Cu(2+), and 188.9mg/g (3633μmol/g) for Cr(3+). The very fast desorption was then achieved using 1.0mol/L HNO3 as the eluent. An enrichment factor of 300 was observed for metal ions adsorbed from solutions containing 2ppb of the metal.
已经通过在 5.5cm 长和 5.6mm ID 的聚丙烯注射器中进行原位聚合，制备了包含各种整体聚合物的用于吸附和预浓缩金属离子的简单装置。用乙二胺对聚(甲基丙烯酸缩水甘油酯-co-二甲基丙烯酸乙二酯)整体柱进行改性，得到螯合材料。首先用光接枝聚甲基丙烯酸丁酯-co-二甲基丙烯酸乙二酯和聚月桂基甲基丙烯酸-co-二甲基丙烯酸乙二酯整体柱，然后用乙二胺进行功能化。或者，还将其他螯合官能团（包括分子量和形状不同的聚亚乙基亚胺（线性和支链）以及溶菌酶）连接到整体支撑物上。我们发现，用乙二胺功能化的聚(甲基丙烯酸缩水甘油酯-co-二甲基丙烯酸乙二酯)整体柱表现出最佳的螯合性能，其特征在于快速吸附以及对 Pb(2+)的容量为 111.2mg/g（537μmol/g），对 Ni(2+)的容量为 38.1mg/g（649μmol/g），对 Cu(2+)的容量为 69.9mg/g（1100μmol/g），对 Cr(3+)的容量为 188.9mg/g（3633μmol/g）。然后使用 1.0mol/L HNO3 作为洗脱液可以实现非常快速的洗脱。从含有 2ppb 金属的溶液中吸附的金属离子的富集因子为 300。
