Didion B A, Graves C N
Department of Animal Sciences, University of Illinois, Urbana 61801.
J Dairy Sci. 1989 Jun;72(6):1540-6. doi: 10.3168/jds.S0022-0302(89)79265-1.
A series of experiments were conducted to determine the role of Ca in several physiological functions of bovine spermatozoa. For spermatozoa incubated in the absence of Ca for up to 24 h, motility was not different from those incubated with Ca. For spermatozoa incubated in the continuous presence of Ca, true acrosome reaction values were 0% at 0 h, 1.5% at 6 h, and 6.0% at 12 h. Spermatozoa incubated in vitro for up to 12 h in the absence of Ca did not undergo a true acrosome reaction; however, when Ca was added during incubation, a synchronous true acrosome reaction was induced within 10 min (0% at 0 h, 8.5% at 6 h, and 8.5% at 12 h). When spermatozoa were preincubated in the presence or absence of Ca for 6 h, then added to zona-intact dead bovine oocytes and incubation continued with and without Ca for 18 h, the number of spermatozoa binding to and penetrating each oocyte was greater when Ca was present. Also, the percentage of oocytes being penetrated was greater when Ca was present. These results indicate that: 1) Ca is not necessary for maintenance of spermatozoan motility; 2) Ca is required for the induction of a true acrosome reaction among a population of spermatozoa; 3) Ca is able to induce the synchronous true acrosome reaction in a low percentage of spermatozoa; and 4) Ca is important in spermatozoan binding and initiation of penetration of oocytes.
进行了一系列实验以确定钙在牛精子几种生理功能中的作用。对于在无钙条件下孵育长达24小时的精子，其活力与在有钙条件下孵育的精子没有差异。对于在持续有钙的条件下孵育的精子，真正的顶体反应值在0小时时为0%，6小时时为1.5%，12小时时为6.0%。在无钙条件下体外孵育长达12小时的精子未发生真正的顶体反应；然而，在孵育过程中添加钙时，在10分钟内诱导了同步的真正顶体反应（0小时时为0%，6小时时为8.5%，12小时时为8.5%）。当精子在有或无钙的条件下预孵育6小时，然后添加到完整透明带的死亡牛卵母细胞中，并在有或无钙的条件下继续孵育18小时时，当有钙存在时，与每个卵母细胞结合并穿透的精子数量更多。而且，当有钙存在时，卵母细胞被穿透的百分比更高。这些结果表明：1）钙对于维持精子活力不是必需的；2）钙是诱导一群精子发生真正顶体反应所必需的；3）钙能够在低比例的精子中诱导同步的真正顶体反应；4）钙在精子与卵母细胞的结合及穿透起始过程中很重要。
