Wedner H J, Wilson P, Lewis W H
Department of Medicine, Washington University School of Medicine, St. Louis, Mo. 63110.
J Allergy Clin Immunol. 1989 Aug;84(2):263-71. doi: 10.1016/0091-6749(89)90334-5.
Parthenium hysterophorus (PH) is a ubiquitous weed found in the U.S. Gulf Coast. In this study, 582 sera, contributed by 22 physicians from 18 Gulf Coast cities, were examined by ELISA for IgE directed against determinants in an aqueous extract of PH pollen, and these were compared to an extract of western ragweed (Ambrosia psilostachya [AP]). Overall, 65.6% of the sera tested were positive for one or both of the pollen extracts examined. Thirty-five percent of the sera were sensitive to PH and 57.6% were sensitive to AP. Thirty percent of the sera were positive to AP only, 8.0% were positive to PH only, and 27.9% of the sera were positive to both extracts. ELISA-inhibition analysis demonstrated that PH and AP extracts contained unique allergenic epitopes. Clinical correlation of in vitro reactivity with skin testing and patients' symptoms confirmed that PH sensitivity by ELISA was related in many cases to fall seasonal symptoms. These data demonstrate by in vitro analysis the presence of IgE directed against an extract of PH pollen in a significant number of patients with fall seasonal pollinosis in the U.S. Gulf Coast. Thus, allergic reactivity to PH may be a significant contributor to fall allergic disease in the U.S. Gulf Coast.
银胶菊是一种在美国墨西哥湾沿岸随处可见的杂草。在本研究中，来自18个墨西哥湾沿岸城市的22名医生提供了582份血清，通过酶联免疫吸附测定法（ELISA）检测针对银胶菊花粉水提取物中决定簇的IgE，并将其与西部豚草（糙果豚草[AP]）提取物进行比较。总体而言，所检测的血清中有65.6%对所检测的一种或两种花粉提取物呈阳性反应。35%的血清对银胶菊敏感，57.6%对西部豚草敏感。30%的血清仅对西部豚草呈阳性，8.0%仅对银胶菊呈阳性，27.9%的血清对两种提取物均呈阳性。酶联免疫吸附抑制分析表明，银胶菊和西部豚草提取物含有独特的过敏原表位。体外反应性与皮肤试验及患者症状的临床相关性证实，在许多情况下，通过酶联免疫吸附测定法检测出的对银胶菊的敏感性与秋季季节性症状有关。这些数据通过体外分析表明，在美国墨西哥湾沿岸，大量秋季季节性花粉症患者体内存在针对银胶菊花粉提取物的IgE。因此，对银胶菊的过敏反应可能是美国墨西哥湾沿岸秋季过敏性疾病的一个重要因素。
