Wuethrich Irene, Guillen Eduardo, Ploegh Hidde L
Whitehead Institute for Biomedical Research , Cambridge, Massachusetts.
Monoclon Antib Immunodiagn Immunother. 2014 Apr;33(2):109-20. doi: 10.1089/mab.2013.0088.
Fluorophores are essential tools in molecular and cell biology. However, their application is mostly confined to the singular exploitation of their fluorescent properties. To enhance the versatility and expand the use of the fluorophore Alexa Fluor 647 (AF647), we generated a mouse monoclonal antibody against it. We demonstrate its use of AF647 for immunoblot, immunoprecipitation, and cytofluorimetry.
荧光团是分子生物学和细胞生物学中的重要工具。然而,它们的应用大多局限于对其荧光特性的单一利用。为了提高荧光团Alexa Fluor 647(AF647)的多功能性并扩大其用途,我们制备了一种针对它的小鼠单克隆抗体。我们展示了AF647在免疫印迹、免疫沉淀和细胞荧光测定中的应用。