Wood P R, Ripper J, Radford A J, Bundesen P G, Rylatt D B, Cottis L E, John M, Plackett P
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
J Gen Microbiol. 1988 Sep;134(9):2599-604. doi: 10.1099/00221287-134-9-2599.
A series of monoclonal antibodies (MAbs), specific for Mycobacterium bovis and BCG strains, were tested extensively for cross-reactivity to a wide range of mycobacterial species using ELISA, Western blotting and dot-blot analysis. The MAbs bound specifically to M. bovis and BCG and showed limited cross-reactivity with some strains of M. tuberculosis. All these MAbs recognized a 22 kDa protein previously termed MPB70, and by competitive ELISA analysis appeared to detect at least three M. bovis-specific determinants on the MPB70 molecule.
一系列针对牛分枝杆菌和卡介苗菌株的单克隆抗体（MAbs），使用酶联免疫吸附测定（ELISA）、蛋白质印迹法和斑点印迹分析，广泛测试了其与多种分枝杆菌物种的交叉反应性。这些单克隆抗体与牛分枝杆菌和卡介苗特异性结合，与某些结核分枝杆菌菌株表现出有限的交叉反应性。所有这些单克隆抗体都识别一种先前称为MPB70的22 kDa蛋白，并且通过竞争性酶联免疫吸附测定分析，似乎在MPB70分子上检测到至少三个牛分枝杆菌特异性决定簇。
