通过基因表达图谱的帽分析对乳腺癌细胞中雌激素调控基因的系统鉴定。
Systemic identification of estrogen-regulated genes in breast cancer cells through cap analysis of gene expression mapping.
机构信息
Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan; Department of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; Department of Anti-Aging Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
出版信息
Biochem Biophys Res Commun. 2014 May 9;447(3):531-6. doi: 10.1016/j.bbrc.2014.04.033. Epub 2014 Apr 16.
To explore the estrogen-regulated genes genome-widely in breast cancer, cap analysis of gene expression (CAGE) sequencing was performed in MCF-7 cells under estrogen treatment. Estrogen-regulated expressional changes were found in 1537 CAGE tag clusters (TCs) (⩾1.5 or ⩽0.66-folds). Among them, 15 TCs were situated in the vicinity of (⩽10 kb) reported estrogen receptor-binding sites. Knockdown experiments of the 15 TC-associated genes demonstrated that the genes such as RAMP3, ISOC1 and GPRC5C potentially regulate the growth or migration of MCF-7 cells. These results suggest that CAGE sequencing will reveal novel estrogen target genes in breast cancer.
为了全面探索乳腺癌中受雌激素调控的基因，我们对 MCF-7 细胞在雌激素处理下的基因表达进行了帽状分析基因表达测序（CAGE 测序）。在 1537 个 CAGE 标签簇（TCs）（≥1.5 或≤0.66 倍）中发现了雌激素调控的表达变化。其中，15 个 TCs 位于报道的雌激素受体结合位点附近（≤10kb）。对 15 个 TCs 相关基因的敲低实验表明，RAMP3、ISOC1 和 GPRC5C 等基因可能调节 MCF-7 细胞的生长或迁移。这些结果表明，CAGE 测序将揭示乳腺癌中新型的雌激素靶基因。
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