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辐射诱导的小鼠胸腺淋巴瘤中p16 DNA甲基化增加。

Increased p16 DNA methylation in mouse thymic lymphoma induced by irradiation.

作者信息

Song Wengang, Liu Yongzhe, Liu Ying, Zhang Cong, Yuan Bao, Zhang Lianbo, Sun Shilong

机构信息

Beihua University, Jinlin, China.

Department of Toxicology, School of Public Health, Tianjin Medical University, Tianjin, China.

出版信息

PLoS One. 2014 Apr 18;9(4):e93850. doi: 10.1371/journal.pone.0093850. eCollection 2014.

DOI:10.1371/journal.pone.0093850
PMID:24747802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3991568/
Abstract

DNA methylation is an important part of epigenetics. In this study, we examined the methylation state of two CpG islands in the promoter of the p16 gene in radiation-induced thymic lymphoma samples. The mRNA and protein levels of P16 were significantly reduced in radiation-induced thymic lymphoma tissue samples. Twenty-three CpG sites of the CpG islands in the p16 promoter region were detected, and the methylation percentages of -71, -63, -239, -29, -38, -40, -23, 46 CpG sites were significantly higher in radiation-induced thymic lymphoma tissue samples than those in matched non-irradiated thymus tissue samples. This study provides new evidence for the methylation state of p16 in the radiation-induced thymic lymphoma samples, which suggests that the methylation of these CpG sites in the p16 promoter may reduce its expression in the thymic lymphoma after irradiation.

摘要

DNA甲基化是表观遗传学的重要组成部分。在本研究中，我们检测了辐射诱导的胸腺淋巴瘤样本中p16基因启动子区两个CpG岛的甲基化状态。辐射诱导的胸腺淋巴瘤组织样本中P16的mRNA和蛋白水平显著降低。检测了p16启动子区CpG岛的23个CpG位点，辐射诱导的胸腺淋巴瘤组织样本中-71、-63、-239、-29、-38、-40、-23、46 CpG位点的甲基化百分比显著高于配对的未照射胸腺组织样本。本研究为辐射诱导的胸腺淋巴瘤样本中p16的甲基化状态提供了新证据，表明p16启动子中这些CpG位点的甲基化可能会降低其在照射后胸腺淋巴瘤中的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/25542c2b3f57/pone.0093850.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/147c3c2a7ab1/pone.0093850.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/0248fdc99798/pone.0093850.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/6f6f14fef3d0/pone.0093850.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/b25c51dd9910/pone.0093850.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/25542c2b3f57/pone.0093850.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/147c3c2a7ab1/pone.0093850.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/0248fdc99798/pone.0093850.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/6f6f14fef3d0/pone.0093850.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/b25c51dd9910/pone.0093850.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5fd/3991568/25542c2b3f57/pone.0093850.g005.jpg

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