Fedotov V p, Baranova I N, Gudoshnikov V I
Probl Endokrinol (Mosk). 1989 May-Jun;35(3):54-8.
The effects of several hormones on total RNA and protein biosynthesis were examined in primary cultures of liver cells obtained from rat fetuses on 21-22 days of gestation and from 3 week-old weanling rats. The intensity of biosynthesis processes was estimated by the incorporation of labeled precursors in macromolecules. Insulin, cortisol, triiodothyronine (T3) stimulated RNA and protein biosynthesis in both types of cultures. These hormones enhanced total protein biosynthesis in fetal rat liver cells more efficiently than in hepatocytes of weanling rats. Somatotropin (growth hormone--GH) did not change total protein biosynthesis but notably increased RNA synthesis and the production of immunoreactive serum albumin. Experiments on fetal rat liver cell cultures showed that stimulating action of cortisol on RNA synthesis was synergistic in relation to the effects of insulin and GH. It has been concluded that fetal rat liver cell at the end gestation are able to respond adequately to anabolic action of the hormones.
研究了几种激素对从妊娠21 - 22天的大鼠胎儿以及3周龄断奶大鼠获得的原代肝细胞培养物中总RNA和蛋白质生物合成的影响。通过将标记前体掺入大分子中来估计生物合成过程的强度。胰岛素、皮质醇、三碘甲状腺原氨酸（T3）刺激了两种类型培养物中的RNA和蛋白质生物合成。这些激素在胎鼠肝细胞中比在断奶大鼠肝细胞中更有效地增强了总蛋白质生物合成。生长激素（GH）没有改变总蛋白质生物合成，但显著增加了RNA合成和免疫反应性血清白蛋白的产生。对胎鼠肝细胞培养物的实验表明，皮质醇对RNA合成的刺激作用与胰岛素和GH的作用具有协同关系。得出的结论是，妊娠末期的胎鼠肝细胞能够对激素的合成代谢作用做出充分反应。
