Department of Pharmacology, Korea University College of Medicine, 126-1, 5-ga, Anam-dong, Seongbuk-gu, Seoul, 136-705, Republic of Korea.
Neurochem Res. 2014 May;39(5):961-72. doi: 10.1007/s11064-014-1294-2. Epub 2014 Apr 20.
Recent study demonstrates antidepressant-like effect of cocaine- and amphetamine-regulated transcript (CART) in the forced swimming test (FST), but less is known about whether antidepressant treatments alter levels of CART immunoreactivity (CART-IR) in the FST. To explore this possibility, we assessed the treatment effects of desipramine and citalopram, which inhibit the reuptake of norepinephrine and serotonin into the presynaptic terminals, respectively, on changes in levels of CART-IR before and after the test swim in mouse brain. Levels of CART-IR in the nucleus accumbens shell (AcbSh), dorsal bed nucleus of the stria terminalis (dBNST), and hypothalamic paraventricular nucleus (PVN) were significantly increased before the test swim by desipramine and citalopram treatments. This increase in CART-IR in the AcbSh, dBNST, and PVN before the test swim remained elevated by desipramine treatment after the test swim, but this increase in these brain areas returned to near control levels after test swim by citalopram treatment. Citalopram, but not desipramine, treatment increased levels of CART-IR in the central nucleus of the amygdala (CeA) and the locus ceruleus (LC) before the test swim, and this increase was returned to control levels after the test swim in the CeA, but not in the LC. These results suggest common and distinct regulation of CART by desipramine and citalopram treatments in the FST and raise the possibility that CART in the AcbSh, dBNST, and CeA may be involved in antidepressant-like effect in the FST.
最近的研究表明可卡因和安非他命调节转录物（CART）在强迫游泳试验（FST）中具有抗抑郁作用，但对于抗抑郁治疗是否会改变 FST 中 CART 免疫反应性（CART-IR）的水平知之甚少。为了探讨这种可能性，我们评估了去甲丙咪嗪和西酞普兰的治疗效果，它们分别抑制去甲肾上腺素和血清素再摄取到突触前末端，观察它们在测试游泳前后对小鼠大脑中 CART-IR 水平变化的影响。去甲丙咪嗪和西酞普兰处理后，伏隔核壳（AcbSh）、终纹床核背侧部（dBNST）和下丘脑室旁核（PVN）中的 CART-IR 水平在测试游泳前显著增加。去甲丙咪嗪治疗后，这些脑区在测试游泳前的 CART-IR 增加仍然升高，但西酞普兰治疗后，这些脑区的 CART-IR 增加在测试游泳后恢复到接近对照水平。西酞普兰治疗而非去甲丙咪嗪治疗可增加测试游泳前杏仁中央核（CeA）和蓝斑核（LC）中的 CART-IR 水平，且 CeA 中的这种增加在测试游泳后恢复到对照水平，但 LC 中未恢复。这些结果表明，去甲丙咪嗪和西酞普兰治疗在 FST 中对 CART 的共同和独特调节，并提出了在 FST 中，AcbSh、dBNST 和 CeA 中的 CART 可能参与抗抑郁作用的可能性。
