Belyaeva N N, Gasimova Z M, Mikhaylova R I, Savostikova O N, Alekseeva A V
Gig Sanit. 2014 Jan-Feb(1):50-4.
There was investigated the dynamics of 1-, 3- and 6-month exposure to 4 doses of silver nanoparticles of size 14.3 +/- 0.05 nm stabilized with gum arabic, and 4 doses of silver sulfate on the liver of male outbred rats by 13 cell morphofunctional indices. As the solvent to obtain a working solution there was used distilled water solutions of different conccentrations were obtained on the base of Moscow tap water cleaned out by a charcoal filter. The animals had free acccess to the drinkers with the studied water. For silver sulfate as a control intact rats served, for silver nanoparticles--acacia gum. The increase in the number of polyploid hepatocytes, micronekroses and discomplexation of hepatic beams and the decreasing the number of reticular endothelial system cells in the liver were shown to permit to evaluate the effect of 6-month nanosilver exposure to the liver at a dose of 0.3 mg/kg as pronounced harmful (Fel), 0.023 mg/ kg--as LOAEL, and 0.0028 and 0.0006 mg/kg--as NOEL. The effect of silver sulfate in doses of 0.28 and 0.03 mg/ kg is assessed as pronounced harmful (Fel), 0,0028 mg/kg--as LAOEL and 0.0005 mg/kg--as NOEL. More earlier detection of toxicity of silver sulfate as Fel (at 3 months) and in smaller doses indicates its greater toxicity to the liver than silver nanoparticles.
通过13项细胞形态功能指标，研究了14.3±0.05纳米大小、用阿拉伯胶稳定的4种剂量银纳米颗粒以及4种剂量硫酸银对雄性远交系大鼠肝脏1个月、3个月和6个月的暴露动态。以蒸馏水作为溶剂来制备工作溶液，不同浓度的溶液是基于经活性炭过滤器净化的莫斯科自来水配制而成。动物可自由饮用含有所研究物质的水。对于硫酸银，以未处理的大鼠作为对照；对于银纳米颗粒，则以阿拉伯胶作为对照。结果显示，多倍体肝细胞数量增加、出现微坏死以及肝梁结构紊乱，同时肝脏中网状内皮系统细胞数量减少，这使得我们能够评估6个月时剂量为0.3毫克/千克的纳米银对肝脏的影响为明显有害（Fel），0.023毫克/千克为最低观察到有害作用水平（LOAEL），0.0028毫克/千克和0.0006毫克/千克为未观察到有害作用水平（NOEL）。剂量为0.28毫克/千克和0.03毫克/千克的硫酸银的影响被评估为明显有害（Fel），0.0028毫克/千克为最低观察到有害作用水平（LAOEL），0.0005毫克/千克为未观察到有害作用水平（NOEL）。硫酸银作为明显有害物（Fel）（在3个月时）且在较小剂量下毒性更早被检测到，这表明其对肝脏的毒性比银纳米颗粒更大。
