Department of Molecular and Clinical Medicine, Institute of Medicine, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.
Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.
PLoS One. 2014 Apr 21;9(4):e95468. doi: 10.1371/journal.pone.0095468. eCollection 2014.
Chronically elevated serum levels of serum amyloid A (SAA) are linked to increased risk of cardiovascular disease. However, whether SAA is directly involved in atherosclerosis development is still not known. The aim of this study was to investigate the effects of adipose tissue-derived human SAA on atherosclerosis in mice. hSAA1+/- transgenic mice (hSAA1 mice) with a specific expression of human SAA1 in adipose tissue were bred with ApoE-deficient mice. The hSAA1 mice and their wild type (wt) littermates were fed normal chow for 35 weeks. At the end of the experiment, the mice were euthanized and blood, gonadal adipose tissue and aortas were collected. Plasma levels of SAA, cholesterol and triglycerides were measured. Atherosclerotic lesion areas were analyzed in the aortic arch, the thoracic aorta and the abdominal aorta in en face preparations of aorta stained with Sudan IV. The human SAA protein was present in plasma from hSAA1 mice but undetectable in wt mice. Similar plasma levels of cholesterol and triglycerides were observed in hSAA1 mice and their wt controls. There were no differences in atherosclerotic lesion areas in any sections of the aorta in hSAA1 mice compared to wt mice. In conclusion, our data suggest that adipose tissue-derived human SAA does not influence atherosclerosis development in mice.
血清淀粉样蛋白 A（SAA）的血清水平持续升高与心血管疾病风险增加有关。然而，SAA 是否直接参与动脉粥样硬化的发展尚不清楚。本研究旨在探讨脂肪组织来源的人 SAA 对小鼠动脉粥样硬化的影响。hSAA1+/−转基因小鼠（hSAA1 小鼠）在脂肪组织中特异性表达人 SAA1，并与 ApoE 缺陷小鼠交配。hSAA1 小鼠及其野生型（wt）同窝仔鼠喂食正常饲料 35 周。实验结束时，处死小鼠并采集血液、性腺脂肪组织和主动脉。测量血浆 SAA、胆固醇和甘油三酯水平。用苏丹 IV 染色的主动脉正面切片分析主动脉弓、胸主动脉和腹主动脉的动脉粥样硬化病变面积。hSAA1 小鼠的血浆中存在人 SAA 蛋白，但 wt 小鼠中无法检测到。hSAA1 小鼠和其 wt 对照组的血浆胆固醇和甘油三酯水平相似。hSAA1 小鼠与 wt 对照组相比，在主动脉的任何节段的动脉粥样硬化病变面积均无差异。总之，我们的数据表明，脂肪组织来源的人 SAA 不会影响小鼠的动脉粥样硬化发展。
