Kim Mi Ha, Rebbert Martha L, Ro Hyunju, Won Minho, Dawid Igor B
Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America.
PLoS One. 2014 Apr 21;9(4):e94873. doi: 10.1371/journal.pone.0094873. eCollection 2014.
March 8 is a member of a family of transmembrane E3 ubiquitin ligases that have been studied mostly for their role in the immune system. We find that March 8 is expressed in the zebrafish egg and early embryo, suggesting a role in development. Both knock-down and overexpression of March 8 leads to abnormal development. The phenotype of zebrafish embryos and Xenopus animal explants overexpressing March 8 implicates impairment of cell adhesion as a cause of the effect. In zebrafish embryos and in cultured cells, overexpression of March 8 leads to a reduction in the surface levels of E-cadherin, a major cell-cell adhesion molecule. Experiments in cell culture further show that E-cadherin can be ubiquitinated by March 8. On the basis of these observations we suggest that March 8 functions in the embryo to modulate the strength of cell adhesion by regulating the localization of E-cadherin.
March 8是跨膜E3泛素连接酶家族的一员，该家族主要因其在免疫系统中的作用而受到研究。我们发现March 8在斑马鱼卵和早期胚胎中表达，这表明其在发育过程中发挥作用。March 8的敲低和过表达都会导致发育异常。过表达March 8的斑马鱼胚胎和非洲爪蟾动物外植体的表型表明，细胞黏附受损是造成这种影响的原因。在斑马鱼胚胎和培养细胞中，March 8的过表达导致主要细胞间黏附分子E-钙黏蛋白的表面水平降低。细胞培养实验进一步表明，E-钙黏蛋白可被March 8泛素化。基于这些观察结果，我们认为March 8在胚胎中通过调节E-钙黏蛋白的定位来调节细胞黏附强度。
