Mohammed Nidhal Abdulmohaimen, Qassem Haitham, Hassen Farouk
Department of Microbiology, Al-Nahrain College of Medicine, Al-Nahrain University, Baghdad, Iraq;
Microbiologist, College of Pharmacy, Al Mustansiyria University, Baghdad, Iraq;
Iran J Med Sci. 2014 Mar;39(2 Suppl):191-5.
Pulmonary tuberculosis (PT) is one of the endemic diseases in Iraq, and among the suggested predisposing factors are alleles of the human leukocyte antigen (HLA) system. We sought to investigate the association between HLA-class I (A and B) and -class II (DR and DQ) alleles in a sample of PT Iraqi patients.
lymphocytes of 105 PT patients and 40 controls were phenotyped for HLA-A, -B, -DR, and -DQ alleles by means of the microlymphocytotoxicity test using a panel of monoclonal antisera.
HLA frequencies of B18 (16.2 vs. 2.5%; OD=7.53) and DR1 (51.4 vs. 10.0%; OD=9.53) alleles were significantly increased in the patients as compared with the controls, while B5 (6.7 vs. 25.0%), DR8 (1.9 vs. 17.5%), and DQ3 (11.4 vs. 45.0%) alleles were significantly decreased. However, a significant corrected level was maintained for only DR1, DR8, and DQ3 alleles (Pc=1.9×10(-5), 0.02 and 1.0×10(-4), respectively).
The results confirm the predisposing and protecting roles of HLA alleles in PT.
肺结核（PT）是伊拉克的地方病之一，人类白细胞抗原（HLA）系统的等位基因是推测的易感因素之一。我们试图在伊拉克肺结核患者样本中研究HLA - I类（A和B）和 - II类（DR和DQ）等位基因之间的关联。
采用一组单克隆抗血清，通过微淋巴细胞毒性试验对105例肺结核患者和40例对照的淋巴细胞进行HLA - A、 - B、 - DR和 - DQ等位基因分型。
与对照组相比，患者中B18（16.2%对2.5%；优势比=7.53）和DR1（51.4%对10.0%；优势比=9.53）等位基因的HLA频率显著增加，而B5（6.7%对25.0%）、DR8（1.9%对17.5%）和DQ3（11.4%对45.0%）等位基因显著降低。然而，仅DR1、DR8和DQ3等位基因维持了显著的校正水平（校正P值分别为1.9×10⁻⁵、0.02和1.0×10⁻⁴）。
结果证实了HLA等位基因在肺结核中的易感和保护作用。
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