Ren Juan, Zhang Yuelang, Cai Hui, Ma Hongbing, Zhao Dongli, Zhang Xiaozhi, Li Zongfang, Wang Shufeng, Wang Jiangsheng, Liu Rui, Li Yi, Qian Jiansheng, Wei Hongxia, Niu Liying, Liu Yan, Xiao Lisha, Ding Muyang, Jiang Shiwen
Cancer Center Xi'an, Shaanxi Province, China.
Department of Imaging, First Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi Province, China.
Int J Clin Exp Med. 2014 Mar 15;7(3):607-15. eCollection 2014.
G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 closely related G protein-coupled receptors. Recent studies have shown that GPR4 plays important roles in angiogenesis, proton sensing, and regulating tumor cells as an oncogenic gene. How GPR4 conducts its functions? Rare has been known. In order to detect the genes related to GPR4, microarray technology was employed. GPR4 is highly expressed in human vascular endothelial cell HMEC-1. Small interfering RNA against GPR4 was used to knockdown GPR4 expression in HMEC-1. Then RNA from the GPR4 knockdown cells and control cells were analyzed through genome microarray. Microarray results shown that among the whole genes and expressed sequence tags, 447 differentially expressed genes were identified, containing 318 up-regulated genes and 129 down-regulated genes. These genes whose expression dramatically changed may be involved in the GPR4 functions. These genes were related to cell apoptosis, cytoskeleton and signal transduction, cell proliferation, differentiation and cell-cycle regulation, gene transcription and translation and cell material and energy metabolism.
G蛋白偶联受体4（GPR4）属于一个由3种密切相关的G蛋白偶联受体组成的蛋白家族。最近的研究表明，GPR4在血管生成、质子感知以及作为致癌基因调节肿瘤细胞方面发挥着重要作用。GPR4是如何执行其功能的？目前所知甚少。为了检测与GPR4相关的基因，采用了微阵列技术。GPR4在人血管内皮细胞HMEC-1中高表达。使用针对GPR4的小干扰RNA来敲低HMEC-1中GPR4的表达。然后通过基因组微阵列分析来自GPR4敲低细胞和对照细胞的RNA。微阵列结果显示，在整个基因和表达序列标签中，鉴定出447个差异表达基因，其中包括318个上调基因和129个下调基因。这些表达显著变化的基因可能参与了GPR4的功能。这些基因与细胞凋亡、细胞骨架和信号转导、细胞增殖、分化和细胞周期调控、基因转录和翻译以及细胞物质和能量代谢有关。
