Ding Li-Feng, Zheng Gang, Yang Jun, Zhou Zhen-Dong, Li Jian-Jun
Zhongguo Gu Shang. 2014 Jan;27(1):48-53.
Polyethylene glycol/bone morphogenetic protein-2 (PEG/BMP-2) nanoparticles were transfected into Rabbit bone mesenchymal stem cells (rBMSCs) and the expression of BMP-2 was detected.
Dissociated rBMSCs were primarily cultured in vitro and BMP-2 gene was transfected into rBMSCs by PEG/BMP-2 nanoparticals and lipofectamine, respectively. The efficiency of transfection was detected by flow cytometry and the expression of BMP-2 was detected by Western Blot and real time RT-PCR.
PEG/BMP-2 nanoparticals were successfully synthesized and transfected into rBMSCs. Compared with the lipofectamine transfection group, PEG/BMP-2 transfection group had higher efficiency and higher BMP-2 expression.
PEG/BMP-2 nanoparticals transfected rBMSCs highly expressed BMP-2,which provided novel strategies for the treatment of bone defect.
将聚乙二醇/骨形态发生蛋白-2（PEG/BMP-2）纳米颗粒转染至兔骨髓间充质干细胞（rBMSCs）中，并检测BMP-2的表达。
将解离的rBMSCs进行体外原代培养，分别用PEG/BMP-2纳米颗粒和脂质体将BMP-2基因转染至rBMSCs中。通过流式细胞术检测转染效率，通过蛋白质免疫印迹法和实时逆转录聚合酶链反应检测BMP-2的表达。
成功合成PEG/BMP-2纳米颗粒并将其转染至rBMSCs中。与脂质体转染组相比，PEG/BMP-2转染组具有更高的转染效率和更高的BMP-2表达。
PEG/BMP-2纳米颗粒转染的rBMSCs高表达BMP-2，为骨缺损的治疗提供了新策略。
