Yan You-Xian, Yao Hong-Bin, Smart Scott E, Mao Li-Bo, Hu Wei, Yuan Shaotang, Du-Thumm Laurence, Masters James G, Yu Shu-Hong, Pan Long
Division of Nanomaterials & Chemistry, Hefei National Laboratory for Physical Sciences at Microscale, Collaborative Innovation Center of Suzhou Nano Science and Technology, Department of Chemistry, University of Science and Technology of China , Hefei 230026, PR China.
Langmuir. 2014 May 13;30(18):5248-55. doi: 10.1021/la500434a. Epub 2014 May 1.
The hierarchical assembly of inorganic and organic building blocks is an efficient strategy to produce high-performance materials which has been demonstrated in various biomaterials. Here, we report a layer-by-layer (LBL) assembly method to fabricate ultrathin hybrid films from nanometer-scale ionic clusters and proteins. Two types of cationic clusters (hydrolyzed aluminum clusters and zirconium-glycine clusters) were assembled with negatively charged bovine serum albumin (BSA) protein to form high-quality hybrid films, due to their strong electrostatic interactions and hydrogen bonding. The obtained hybrid films were characterized by scanning electron microscope (SEM), UV-vis, Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray fluorescence (XRF), and X-ray diffraction (XRD). The results demonstrated that the cluster-protein hybrid films exhibited structural homogeneity, relative transparency, and bright blue fluorescence. More importantly, these hybrid films displayed up to a 70% increase in hardness and up to a 100% increase in reduced Young's modulus compared to the pure BSA film. These hybrid cluster-protein films could be potentially used as biomedical coatings in the future because of their good transparency and excellent mechanical properties.
无机和有机结构单元的分层组装是制备高性能材料的有效策略，这已在各种生物材料中得到证实。在此，我们报道了一种逐层（LBL）组装方法，用于由纳米级离子簇和蛋白质制备超薄混合膜。两种阳离子簇（水解铝簇和锆 - 甘氨酸簇）与带负电荷的牛血清白蛋白（BSA）蛋白质组装，由于它们之间强烈的静电相互作用和氢键作用，形成了高质量的混合膜。通过扫描电子显微镜（SEM）、紫外可见光谱、拉曼光谱、傅里叶变换红外光谱（FTIR）、X射线荧光（XRF）和X射线衍射（XRD）对所得混合膜进行了表征。结果表明，簇 - 蛋白质混合膜表现出结构均匀性、相对透明度和亮蓝色荧光。更重要的是，与纯BSA膜相比，这些混合膜的硬度提高了70%，约化杨氏模量提高了100%。由于其良好的透明度和优异的机械性能，这些混合簇 - 蛋白质膜未来有可能用作生物医学涂层。
