Azad Md Abul Kalam, Amin Latifah, Sidik Nik Marzuki
Centre for General Studies, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia ; Department of Agricultural Extension, Khamarbari, Farmgate, Dhaka 1215, Bangladesh.
Centre for General Studies, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia.
ScientificWorldJournal. 2014 Mar 17;2014:768038. doi: 10.1155/2014/768038. eCollection 2014.
Papaya (Carica papaya) is severely damaged by the papaya ringspot virus (PRSV). This review focuses on the development of PRSV resistant transgenic papaya through gene technology. The genetic diversity of PRSV depends upon geographical distribution and the influence of PRSV disease management on a sequence of PRSV isolates. The concept of pathogen-derived resistance has been employed for the development of transgenic papaya, using a coat protein-mediated, RNA-silencing mechanism and replicase gene-mediated transformation for effective PRSV disease management. The development of PRSV-resistant papaya via post-transcriptional gene silencing is a promising technology for PRSV disease management. PRSV-resistant transgenic papaya is environmentally safe and has no harmful effects on human health. Recent studies have revealed that the success of adoption of transgenic papaya depends upon the application, it being a commercially viable product, bio-safety regulatory issues, trade regulations, and the wider social acceptance of the technology. This review discusses the genome and the genetic diversity of PRSV, host range determinants, molecular diagnosis, disease management strategies, the development of transgenic papaya, environmental issues, issues in the adoption of transgenic papaya, and future directions for research.
番木瓜（番木瓜属）受到番木瓜环斑病毒（PRSV）的严重损害。本综述聚焦于通过基因技术培育抗PRSV转基因番木瓜。PRSV的遗传多样性取决于地理分布以及PRSV病害管理对一系列PRSV分离株的影响。病原体衍生抗性的概念已被用于转基因番木瓜的培育，采用外壳蛋白介导、RNA沉默机制以及复制酶基因介导的转化来有效管理PRSV病害。通过转录后基因沉默培育抗PRSV番木瓜是一种有前景的PRSV病害管理技术。抗PRSV转基因番木瓜对环境安全，对人类健康无有害影响。近期研究表明，转基因番木瓜的推广成功取决于其应用情况、是否为商业可行产品、生物安全监管问题、贸易法规以及该技术在更广泛范围内的社会接受度。本综述讨论了PRSV的基因组和遗传多样性、寄主范围决定因素、分子诊断、病害管理策略、转基因番木瓜的培育、环境问题、转基因番木瓜推广中的问题以及未来研究方向。
