Wan D C, Livett B G
Department of Biochemistry, University of Melbourne, Parkville, Vic., Australia.
Neurosci Lett. 1989 Jun 19;101(2):218-22. doi: 10.1016/0304-3940(89)90534-x.
The effects of vasoactive intestinal peptide (VIP) and substance P (SP) on the amount of proenkephalin A (ProEnk A) mRNA in cultures of bovine adrenal chromaffin cells were examined. Exposure of chromaffin cells to 5 microM VIP for 24 h produced a significant elevation in ProEnk A mRNA. The stimulatory effect of VIP could be abolished by the presence of the calcium channel blocker D600 or actinomycin D but was not affected by the nicotinic antagonist hexamethonium. The results suggest that VIP may induce transcription of ProEnk A mRNA by a Ca2+-dependent, non-cholinergic mechanism. By contrast, SP (5 microM) had no effect on the amount of ProEnk A mRNA. Since VIP is found in nerve terminals and the ganglion cells within the adrenal medulla, this peptide could be an endogenous regulator of adrenal enkephalin gene expression.
研究了血管活性肠肽（VIP）和P物质（SP）对牛肾上腺嗜铬细胞培养物中前脑啡肽原A（ProEnk A）mRNA含量的影响。将嗜铬细胞暴露于5微摩尔VIP 24小时，可使ProEnk A mRNA显著升高。VIP的刺激作用可被钙通道阻滞剂D600或放线菌素D消除，但不受烟碱拮抗剂六甲铵的影响。结果表明，VIP可能通过钙依赖性、非胆碱能机制诱导ProEnk A mRNA的转录。相比之下，SP（5微摩尔）对ProEnk A mRNA的含量没有影响。由于在肾上腺髓质内的神经末梢和神经节细胞中发现了VIP，这种肽可能是肾上腺脑啡肽基因表达的内源性调节因子。
