Jahan Sarwat, Zahra Asia, Irum Umaira, Iftikhar Natasha, Ullah Hizb
Reproductive Physiology Laboratory, Department of Animal Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
Syst Biol Reprod Med. 2014 Aug;60(4):199-205. doi: 10.3109/19396368.2014.912363. Epub 2014 Apr 23.
The present study was performed to determine the effects of different antioxidants on testicular histopathology and oxidative damage induced by cadmium (Cd) in rat testis and prostate. Twenty five rats were equally divided into five groups (n = 5/group). The control group was injected subcutaneously with saline while the Cd alone treated group received a subcutaneous injection of 0.2 mg/kg CdCl(2). Other groups were treated with sulphoraphane (25 µg/rat), vitamin E (75 mg/kg), and Ficus Religiosa plant extract (100 mg/kg) orally along with subcutaneous injections of 0.2 mg/kg CdCl(2) for fifteen days. Oxidative damage in the testicular and prostate tissues were assessed by the estimation of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and glutathione reductase (GSR) activity. Lipid peroxidation (TBARS), protein estimation, and histomorphology were also assessed. Cadmium exposure caused a significant decrease in antioxidant enzymes like CAT, POD, SOD, GSR, protein concentrations, and a marked increase in TBARS activity in rat testis and prostate. Histological examination of adult male rat testes showed a disruption in the arrangement of seminiferous tubules along with a reduction in the number of germ cells, Leydig cells, tunica albuginea thickness, diameter of seminiferous tubules, and height of germinal epithelium. Co-treatment with vitamin E, sulphoraphane, and Ficus religiosa were found to be effective in reversing Cd induced toxicity, representing potential therapeutic options to protect the reproductive tissues from the detrimental effects of Cd toxicity.
本研究旨在确定不同抗氧化剂对镉（Cd）诱导的大鼠睾丸和前列腺组织病理学及氧化损伤的影响。将25只大鼠平均分为五组（每组n = 5）。对照组皮下注射生理盐水，而单独镉处理组皮下注射0.2 mg/kg CdCl₂。其他组在皮下注射0.2 mg/kg CdCl₂的同时，口服萝卜硫素（25 μg/只）、维生素E（75 mg/kg）和无花果植物提取物（100 mg/kg），持续15天。通过测定过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）和谷胱甘肽还原酶（GSR）的活性来评估睾丸和前列腺组织中的氧化损伤。还评估了脂质过氧化（TBARS）、蛋白质含量及组织形态学。镉暴露导致大鼠睾丸和前列腺中抗氧化酶如CAT、POD、SOD、GSR及蛋白质浓度显著降低，TBARS活性显著升高。成年雄性大鼠睾丸的组织学检查显示，生精小管排列紊乱，生殖细胞、睾丸间质细胞数量减少，白膜厚度、生精小管直径及生精上皮高度降低。发现维生素E、萝卜硫素和无花果联合处理可有效逆转镉诱导的毒性，为保护生殖组织免受镉毒性的有害影响提供了潜在的治疗选择。
