Shikhman A R, Cherniakova A M, Shmidt E I, Briko N I
Vestn Akad Med Nauk SSSR. 1989(6):46-9.
A sandwich ELISA system was used for the study of the identification of antigenic group A polysaccharide determinants by antibodies in a suspension of streptococcus cells with varying degrees of lysis. Antibodies were able to identify less than 0.01% of antigenic A polysaccharides determinants in intact streptococcal cells. It is concluded that A polysaccharide is a marker of the destroyed group A streptococcus cells. The circulation of group A polysaccharide was examined in the sera of patients with rheumatism and acute streptococcal tonsillitis. Group A polysaccharide was detectable more frequently in rheumatic patients, as compared to those with tonsillitis, and circulated for long periods of time in the vascular network. Avidity and titres of antibodies to group A polysaccharide were determined in rheumatic patients with different degrees of antigenemia. In cases of marked antigenemia, correlation was disrupted between the titre and avidity of antibodies to group A polysaccharides.
采用夹心酶联免疫吸附测定（ELISA）系统，研究在不同程度裂解的链球菌细胞悬液中，抗体对A群多糖抗原决定簇的识别情况。抗体能够识别完整链球菌细胞中不到0.01%的A群多糖抗原决定簇。由此得出结论，A群多糖是被破坏的A群链球菌细胞的标志物。对风湿热患者和急性链球菌性扁桃体炎患者血清中的A群多糖进行了检测。与扁桃体炎患者相比，风湿热患者血清中A群多糖的检出频率更高，且在血管网络中循环较长时间。测定了不同程度抗原血症的风湿热患者体内抗A群多糖抗体的亲和力和滴度。在抗原血症明显的病例中，抗A群多糖抗体的滴度与亲和力之间的相关性被破坏。
