Cosar Murat, Kaner Tuncay, Sahin Onder, Topaloglu Naci, Guven Mustafa, Aras Adem Bozkurt, Akman Tarık, Ozkan Adile, Sen Halil Murat, Memi Gulsum, Deniz Mustafa
Department of Neurosurgery, Canakkale 18 March University, ,, Turkey.
Department of Neurosurgery, Medeniyet University, Istanbul, Turkey.
Acta Cir Bras. 2014 Apr;29(4):268-73. doi: 10.1590/s0102-86502014000400008.
To investigate the neuroprotective effects of Sulindac on the hippocampal complex after global cerebral ischemia/reperfusion (I/R) injury in rats.
Thirty one Sprague-Dawley rats were used, distributed into group I (sham) n:7 were used as control. For group II (n:8), III (n:8) and IV (n:8) rats, cerebral ischemia was performed via the occlusion of bilateral internal carotid artery for 45 minutes and continued with reperfusion process. 0.3 mL/kg/h 0.9 % sodium chloride was infused intraperitoneally to the Group II rats before ischemia, 5μg/kg/h/0.3 ml sulindac was infused intraperitoneally to the Group III rats before ischemia and 5μg/kg/h/0.3 ml sulindac was infused intraperitoneally to the Group IV rats after ischemia and before reperfusion process. The levels of MDA, GSH and MPO activity were measured in the left hippocampus tissue. The hippocampal tissue of all group members were taken for histopathological study.
The MDA and MPO levels increased from group I (control) to group II (I/R) (P<0.05) and decreased from group II (I/R) to group III (presulindac + I/R) and IV (postsulindac + I/R) (P<0.05). Beside these, the GSH levels decreased from group I (control) to group II (I/R) (P<0.05) and increased from group II (I/R) to group III (presulindac + I/R) and IV (postsulindac + I/R) (P<0.05).The number of apoptotic neurons increased from group I (control) to group II (I/R) (P<0.05) and decreased from group II (I/R) to group III (presulindac + I/R) and IV (postsulindac + I/R) (P<0.05).
The Sulindac may have neuroprotective effects on ischemic neural tissue to prevent the reperfusion injury after ischemia.
研究舒林酸对大鼠全脑缺血/再灌注（I/R）损伤后海马复合体的神经保护作用。
使用31只Sprague-Dawley大鼠，分为I组（假手术组），n = 7只作为对照。对于II组（n = 8只）、III组（n = 8只）和IV组（n = 8只）大鼠，通过阻断双侧颈内动脉45分钟进行脑缺血，并持续进行再灌注过程。II组大鼠在缺血前腹腔注射0.3 mL/kg/h的0.9%氯化钠，III组大鼠在缺血前腹腔注射5μg/kg/h/0.3 ml舒林酸，IV组大鼠在缺血后再灌注前腹腔注射5μg/kg/h/0.3 ml舒林酸。测量左侧海马组织中丙二醛（MDA）、谷胱甘肽（GSH）水平和髓过氧化物酶（MPO）活性。取所有组成员的海马组织进行组织病理学研究。
MDA和MPO水平从I组（对照组）到II组（I/R）升高（P<0.05），从II组（I/R）到III组（舒林酸预处理 + I/R）和IV组（舒林酸后处理 + I/R）降低（P<0.05）。此外，GSH水平从I组（对照组）到II组（I/R）降低（P<0.05），从II组（I/R）到III组（舒林酸预处理 + I/R）和IV组（舒林酸后处理 + I/R）升高（P<0.05）。凋亡神经元数量从I组（对照组）到II组（I/R）增加（P<0.05），从II组（I/R）到III组（舒林酸预处理 + I/R）和IV组（舒林酸后处理 + I/R）减少（P<0.05）。
舒林酸可能对缺血神经组织具有神经保护作用，以预防缺血后的再灌注损伤。
